Dub Turbo Download

Are You Looking For Dub Turbo Torrent? The Dub Turbo torrent has been the most widely searched torrent on the internet recently. The latest sensation in the music industry is the Dub Turbo software which is a user-friendly software aimed at helping musicians who are looking to create their own music. It allows musicians to experiment with mixing beats and creating quality tunes. The best part of the software is that it is not only for professional use and even beginners can use it because it includes video tutorials in laymen terms.Why Are The Masses Looking For A Dub Turbo Torrent? Torrents are the most widely used peer-to-peer file sharing technique. The peer-to peer file distribution system consists of a wide network of people who share files through a peer-to-peer file sharing software via torrents. Torrents can be used to download various softwares, books, music and other online material. Most of the people are looking for a Dub Turbo torrent these days. This is mainly because t hey want to save up money and get the software for free.Some also argue that they want to try it for free before paying for the software, but then again, what is the free 60 day trial included in the original package for? Where to get a Dub Turbo Torrent From? A Dub Turbo torrent is easily available on the internet. The torrent file can be downloaded and a peer-to-peer software can be used to enable Dub Turbo torrent download from the host site. But in order to make the right choices, it is a must that one reads all the reviews before taking a step. It is important to know what other people have to say about the torrent file before you download it.What to expect from a Dub Turbo torrent? There is a high probability that a Dub Turbo torrent free download may not contain the full features of the original package. Also, it does not provide the facility of automatic updates that the paid for Dub Turbo software provides. It is vital that one is aware of the fact that a free download may come with virus, spyware and malware which can harm your PC. In addition to all this, the quality of the pirated software may not be up to the mark and might be a cause of major disappointment. Poor video quality is just one example. What Is The Alternate To A Dub Turbo Torrent?A free download for a software that is available for as low as $50 does not seem just on ethical and legal grounds, to say the least. It is not morally right to steal a software the producers of which have spent precious time and money on developing. It is a way better option to buy the software instead as that would save you from the legal hassle related to copyrights and will also provide full features of the software. The original software also contains automatic updates which do not come with the pirated one. When all of this is available at a nominal rate and with a free 60 day trial, who needs a Dub Turbo torrent?

Real Beauty Sketches Essay

In April 2013, Dove launched a â€Å"Real Beauty† campaign after performing marketing research and discovering that only 2% of all women consider themselves to be â€Å"beautiful†. In an effort to boost self-esteem and moral, Dove introduced a television ad in the form, which incorporated a powerful and touching social experiment. In this commercial, several different women are asked to describe their physical appearance to a forensic sketch artist, who cannot see them as he sketches their physical descriptions. They are told to use neutral terms, and to â€Å"stick to the facts† when describing their own self-reflections. Next, the same women are described to the same forensic sketch artist by a complete stranger, whom they had only met the day before. The strangers are given the same instructions. At the end of this unknown experiment, both descriptions are drawn by the artist and then compared. Upon comparison, it is incredibly obvious that the stranger’s physical descriptions of the subject are more flattering, and more accurate depict the subject’s true appearance. The commercial sends a very inspiring, real message to the women: to be less critical of yourself, and to embrace the natural beauty of womanhood. After the experiment took place, one woman admitted, â€Å"I should be more grateful of my natural beauty. It impacts the choices and the friends we make; the jobs we apply for; how we treat our children; it impacts everything. It couldn’t be more critical to your happiness. † To the same point, another woman added, â€Å"we spend a lot of time as women analyzing and trying to fix the things that aren’t quite right, and we should spend more time appreciating the things that we do like. † The commercial ends with a powerful and honest message: â€Å"You are more beautiful than you think. † The â€Å"Real Beauty Sketches† ad resulted in widespread reactions worldwide- some positive, some negative. Many praised the commercial for its ability to acknowledge and accept natural beauty and what society may traditional deem as physical â€Å"flaws†. But there were many negative critics as well. Despite the good nature behind the commercial’s intent, many criticized the ad for its heavy emphasis on physical appearance. Critics claimed that Dove’s advertisement still focused too heavily on the physical appearance of woman, and its remaining influence as the single-most defining element of a woman’s worth; as opposed to her personality or personal achievements. Which led me to question: How could this advertisement be improved to focus on the â€Å"true beauty† and essence of a woman’s worth? The message is a refreshing change from the typical message that beauty supply companies often send; instead of shaming women into purchasing things to make them more â€Å"beautiful†, Dove takes a non-traditional approach by supporting the natural beauty, without the enhancement of material products. Although this may seem to counter-act their ultimate goal of selling beauty products, Dove gains seeks to gain more than just revenue- they seek to gain self-confidence and acceptance. But in order to overcome the heavy emphasis of a woman’s physical appearance as the defining element of a woman’s worth, I think it is important to emphasis how the woman achieved her â€Å"beauty lines†, wrinkles, or â€Å"flaws†. Perhaps pulling on the viewer’s emotional heartstring of laughter and love would prove to play a beautiful part in the development of a woman’s wrinkles around her eyes, or expression lines in her forehead. By approaching the beauty from this marketing angle, marketers are focusing on the internal beauty that a woman envelopes, and justifies their natural beauty with self-worth and personal accomplishment. By casting very â€Å"average† and â€Å"typical† looking women, marketers sought to appeal to the majority of women, in an effort to reflect a more realistic depiction of women. By refraining from using â€Å"traditionally† beautiful women, viewers and women find the message to be more relatable to themselves. Marketing Applications: In April 2013, Dove marketers applied a story-like short film to their Real Beauty campaign, in an effort to reveal each woman’s true natural beauty. Marketing Relevance: Marketing emphasis: to encourage the improvement of self-image and confidence among women worldwide. Dove marketers sought to prove that â€Å"we† are our own worst critics, and that our own self-image is often skewed and far less beautiful than our true natural beauty suggests. Intangible elements: Dove focused on producing a moral campaign that reflects the ideals and beliefs of their corporate personnel. In this powerful, thought-provoking message, Dove effectively used an emotional story to â€Å"make a culturally positive difference†. Marketers drew upon an emotional appeal in an attempt to attach these positive ideas to their own product line. In doing so, Dove successfully attached an extension of their own feelings to their product, as well as their corporate image. Many accredit the campaign’s success to its ability tap into deep-seated emotions and â€Å"hit on a real human truth for women. Many women undervalue themselves and also the way they look. † In this ad, Dove focused on the emotional appeal, rather than simply â€Å"communicating a particular product or service benefit. † As a result, consumers will be more likely to remember the positive emotions that the advertisement evoked, and thus, â€Å"associate them with Dove products. † Discussion Questions:

Case for critical thinking: A flood of decisions Essay

1. What information sources (or potential information sources) could have been used to assist with the decision-making process for Wivenhoe Dam in this case? Potential information sources that could have been used to assist with the decision-making process for Wivenhoe Dam -SEQWater -Sought advice from Water Grid Manager -Water Commission -DERM (Department of Environmental Resource Management) Managerial decision-making Problem avoiders Problem solvers Problem seekers Approaches to decision making Classical decision model Behavioral decision model Judgmental decision model 2. With references to decision-making theory covered in the chapter, describe the type of managerial decision-making evident in this case, and the conditions under which decisions were made. Managerial Decision Making *Problem avoidably *Problem solvers *Problem seekers Decision conditions: *Certain environment *Risk environment *Uncertain environment 3. Evaluate the decisions made in the case in relation to the classical, behavioural and judgmental heuristics approaches to decision-making that are outlined in the chapter. Which model do you believe best describes the situation and subsequent decision-making process in this case? Justify your answer? Approaches to decision making ^Classical decision model ^Behavioral decision model ^Judgmental decision model Case decision *Classical decision model Problem: it was the flood that damage Brisbane and Ipswich Possible alternative: Not releasing flood waters. Consequently threatened stability of dam Optimizing decision: Release of flood waters being aware of potential damage. SWOT Analysis: Strengths: *New technology *They set priorities *Manage time Weakness: Lack of communication Misunderstanding Misconduct Problem solving Crisis Opportunities: *Professional engineer *Employment Threats: Natural disaster (climate change) Damage roads and homes. Conclusion People should make wise decision to save the lifes of the others.

Analytical critique of Killer Angels by Michael Shaara Essay

Analytical critique of Killer Angels by Michael Shaara - Essay Example Shaara has largely referred to Harrison, who was an actor whom General Longstreet had selected as a spy to gather the consents of the Union forces. Harrison has been used in the book as a narrator, thus neutralizing the approach of Shaara in narration of the events preceding, during and after the battle. Maintaining extreme neutrality, Shaara has conveyed the planning of Union forces as well as that of the Confederate. The story gains its start from June 1863. Third summer during the American Civil War was in season and only few days were left to the commencement of the bloodiest battle in the history of America, the battle of Gettysberg. â€Å"Often referred to as the â€Å"High Water Mark of the Rebellion†, it was the war's bloodiest battle with 51,000 casualties and the setting for President Abraham Lincoln's â€Å"Gettysburg Address†Ã¢â‚¬  (National Park Service, 2011). It was the deadliest and the largest battle among all fought on the land of America. 120 rebell ious men were given under the command of the Colonel Joshua Lawrence Chamberlain who was already commanding the 20th Maine. Unlike the other men of the regiment who had signed contracts for two years, these mutinous men that had freshly been given under the command of the Colonel signed contracts for three whole years. Therefore, these 120 men had to undergo great emotional stress as they saw other men leave for home after two years of service. These men were penalized and were starved. They were all brought to Chamberlain who was allowed to murder anyone who would not willfully go back for fighting. Prior to joining the war, Colonel Chamberlain had remained a faculty member in a college in US. Having come from an educative background, Chamberlain was quite considerate and listened to the concerns of the men. He was against the view of forcing anyone into the fight of freedom and thought of such concepts as ironic and disgusting. Therefore, Chamberlain remained kind enough to promis e the men that he would address their concerns once the Battle of Gettysberg was over. Although Chamberlain considered loss of the Battle of Gettysberg loss of the whole Civil War, yet he provided the men with the choice of participating in or retreating from the battle. However, Chamberlain did adopt a fairly reasonable and educated approach to convince the men to participate whole heartedly in the war. He took the men into confidence and told them that even if their fight was not meant for acquisition of any power or land, their participation in the war would serve the noble cause of providing others with freedom. He told the men that if they won the battle with their effort and participation, it would bring a significant change in the society and the people would be credited for their hard work, and not for their belongingness or race, cast or creed. Chamberlain remained so humble and considerate throughout in his dealing and interaction with the mutinous men that all except for six of them acceded to his opinions and agreed to follow his directions. General Robert E. Lee happened to be an extremely flexible leader who would make abrupt changes in plans and reintroduce fresh strategies every time, he felt things were not going the way they should. Like Chamberlain, General Lee also maintained a very humble character who refused to gamble, drink, or curse. Shaara has

Sadown Analysis Paper Draft 1 Essay Example | Topics and Well Written Essays - 1000 words

Sadown Analysis Paper Draft 1 - Essay Example Not because a black cat passes by it means that bad luck is coming to that person. Signs should only be a guide on how we would live and not the basis why we are living. This is the sad part because it is very common that signs are limiting the decision making of people and influencing how they are living. Examples are fortune tellers for example the palm readers who influences people by reading palms and the lines in a person’s hand to tell their loyal customers the future and their fortunes and misfortunes. This is the big misconception about signs (73). Schnackenberg wrote in her poem the simplest signs that people misunderstand. There are no unlucky things in this world. A person should be knowledgeable enough to either have a free will and know what should and should not be done or be deceived by worldly beliefs. When the author wrote of the signs it did not mean that each sign should be observed by all or would hold the same meaning for everyone. There are things in the environment that has been observed to be true throughout the years. The author gave an example on the second stanza of the poem. These things are uncontrollable and are nature’s own way of living. This does not make any difference in a person’s luck or life. Though the course of nature affects humans one way or another, it is not the reason for humanity’s triumph or destruction. These natures’ occurrence can help mankind in telling the weather, season and time. It is helpful in some aspects of man’s daily life but not to control man’s free will and decision making (73). Beliefs in superstitious signs are fast growing amidst the vast growth of the influence of Science and Technology in the youth. As an author, Schnackenberg knows about the growth of followers at the end of the last stanza. Quite peculiar is that there are so many things that Science can explain and justify yet mass of people from different walks of life are still believing in superstition. Not

Abortion laws in the state of Ohio Term Paper Example | Topics and Well Written Essays - 750 words

Abortion laws in the state of Ohio - Term Paper Example The state encourages the practice of a compulsive delay of 24 hours prior to the abortion (Laws). This 24 hour period is to be used for the purpose of state-directed counseling (Laws). This piece of counseling information must be handed over to the licensed physician, who is going to conduct the operation (Laws). This 24 hour consent law even makes it mandatory for a woman to give some information to the physician upon a face to face meeting (Laws). The physician must retrieve the following information from the patient:- (Laws). It is only after all the procedures are completed, that the patient gets to sign an informed consent (Laws). The patient even has to declare that she is not under the influence of any drugs or alcohol at that point of time (Laws). If a patient is less than 18 years of age, a parent, legal guardian or custodian must go along with her to the pre-abortion visit (Laws). The law even requires that the parent/legal guardian/custodian, give their Informed Consent on the abortion (Laws). From the above discussion we can conclude that the abortion laws in the state of Ohio have been designed by the government with the intention of protecting the innocent fetus from the brutal process of abortion. The laws have been formulated keeping in mind the complete safety of the patients. Also, the requirement of parent’s consent criteria sounds veritably justified enough for a healthy and happy society. Sweigart, Josh. â€Å"Area representative at center of Ohio abortion controversy†. Springfield News-Sun. 27 Mar. 2011. 28 Mar. 2011. OKeefe, Paul. â€Å"Proposed Ohio abortion law would be one of strictest in country†. Wews Newsnet5.com. 8 Feb. 2011. 28 Mar. 2011.

Expansion Through Franchising Case Study Example | Topics and Well Written Essays - 1250 words - 7

Expansion Through Franchising - Case Study Example With the expansion and maturity of the franchise, the chain management often buys them or became interested in opening own stores for themselves which help in gaining a better understanding and controlling of the operations. And with increasing control and understanding the franchising fees get supplemented by profits. Secondly, the decision to choose the town, the neighborhood and even which side of the street as the region of the location is an important key factor of success. A difference in the location of the store can make a huge difference between the success and the failure. Thus the importance of ‘scale economies’ is to be recognized at the store level because the fixed cost of the outlets are very high and so to meet the cost, the small returns per unit cell and the traffic volume is very crucial. Efficiency in store management is also an important factor of profitability within the industry. Since the opportunities of waste, inefficiency, and shrinkage are man y there is a requirement of an efficient management system. Thirdly the efficiency of the store managers in building relations with the local public, developing goodwill with the customers, maintenance of the morality of the employees and keeping an eye on the competing chains are important for ensuring the short-term profits. Fourthly with the development of the industry, a clear overall market image of the chain is very important. There is a need for maintaining consistency and reliability of the product line throughout the chain. Introducing more innovative recipes often becomes risky as it may deviate from the basic and core theme of the industry. (Bartlett and Rangan, 2) Dick Mayer emphasizes on Strategic planning. According to him strategic planning can bring effective changes in the way of marketing and brings accountability in the function. Every product and their pricing proposals require analysis and review by the management to evaluate their ability to meet long-term strategies. He focused mainly on the subsidiary in Japan as it was one of the fastest  growing units with the highest potentiality also a challenge for the entire international operations.  

Dissertation Topic in Financial Management Essay

Dissertation Topic in Financial Management - Essay Example Markets have become globalised, yet tax structures have remained national. Open borders cause exaggerated tax competition, which in turn leads to a race to the companies. Transparency of financial markets means correct management of all information related to capital flows that also include full tax revenue. Although developing countries have often been reprimanded for their insufficient and malfunctioning financial and tax systems, they are not the only guilty parties for the failure of system. There is a vital activity from both on a national and international scale is the necessary task to control the general loss of revenue. International tax competition and harmful tax practices are providing more and more opportunities for some parties to escape their tax obligations. In this scenario the burden of taxation will be shifted to some other segments like ordinary citizens. The issue of additional clarity and freedom in exchanges of information between countries with regards to tax matters has become necessary to help many tax administrations to deal effectively in the context of the globalization of world economies. Hence the current study proposes to identify the tax scenario in the global finance markets Also the study intends to observe the global initiative and the regulations to clear out the and the disparities between the ability of developed and developing countries to obtain and provide the required information for the effective control of harmful tax competition and tax evasion issues. By doing such study the current research intends to make some recommendations to strengthen the current tax system of global financial market. Research Problem Area: Tax issue in the global finance markets. Research Objectives: To observe the scenario of global financial markets To identify the tax issues in the international trade To analyze the regulations from national and international bodies To make recommendations to strengthen the tax system Research approach: The research study of 'Tax issue in the global finance markets' is proposed to follow Qualitative study methodology.. Data collection method: The Data collection is proposed mainly through Secondary data (research & opinion) and Observation of status of international trade and tax system and the regulations and policy framework. Key areas Associated with the Research: The study intends to observe the literature review on the following aspects 1. Overview of International financial markets The foreign exchange market "Offshore" markets Offshore financial centres International banking and "global trading" 2. The globalization of financial markets: theory and practice Financial markets' integration The different flows of capital Regulatory environment The government's role in financial markets 3. Taxing issue: Requirements of Tax system for international finance market Taxing capital in a multinational environment: tax competition, tax coordination and tax harmonization Tax competition: a virtue or a problem 4. Tax Evasion Global challenges of Tax evasion Relationship between tax rates and tax evasion Electronic payments and taxing Offshore tax evasion Tax heavens Tax shelters Models to incorporate tax evasion Money laundering and

The Invention of Electricity Changed the Way People Live Their Lives Essay

The Invention of Electricity Changed the Way People Live Their Lives - Essay Example It was not until 1792 when a thunderstorm struck Benjamin Franklins home, and he decided to make an experiment by flying the kite through the thunderstorm with an attached iron key. The kite was struck by lightning, and electric current were sent down to Benjamin’s hand. He was  quite  lucky to survive, and he stated that electricity existed in the thunderstorm but the question was how to tap and utilise the electricity for the benefit of humanity. Before the invention of electricity, people had difficulties in performing the several task in life, there was too much utilization of man energy in different sectors. However, the invention of electricity changed the lives of human beings in thousand ways. It can be stated that electricity led to industrial revolution in which several products were being produced in large numbers. It led to a change in the entertainment sectors, food sector health sector and communication sector among others. It is imperative to note that the invention of electricity resulted in a significant improvements of how human beings live in the today’s world. Before the invention of electricity, the period was being described as the era of the dark ages because people relied only on sunlight to perform their daily activities and at night, they were unable to perform duties  because of the darkness. The invention of light electricity has led to the robust influence of how people carry out their lives in the current period. Thomas Edison who was an American inventor as well as a businessperson is thought to be the first person who invented light bulb that was using direct current electricity. The light bulb that was invented had a great influence on how people were carrying business all over the world. Moreover, it led to the inventions of other devices such as televisions and phonographs that were being used as the means of communication (Freeberg,

Week5 Article Example | Topics and Well Written Essays - 500 words

Week5 - Article Example In this article, the author argues that public representation is a worthwhile aim of public policy because public service has always been a driver of social mobility for groups that have been excluded in labor market. Further, it means a symbolic commitment equal access to authority or power. In addition, the author argues that bureaucracy should be more reflective of the economic status of citizens than elected officials since it has an influential role in the policy making process. Active representation is also significant because the operations and policies of diverse institutions will appear distinctively from what they would be if the institution’s employee were homogeneous (Selden, 2006). The United States has always been on the forefront in search of a diverse workforce via an emphasis on affirmative action policies and equal opportunity. To some proponents, moving away from affirmative action as the main tool for bringing both active and passive representation in the U.S would go against the constitutional principles of the U.S constitution. They argue that it is irrational to ignore the ugly remnants of ethnicity, race, and gender in the recruitment, promotion and retention decisions in the private, non-profit and public agencies of the U.S. Research conducted by Gallup in 2005 showed that through affirmative action, minority rights and relations were defended since affirmative action calls for the rights of racial minorities. To some opponents, affirmative action is viewed as an equally division model of reverse biasness or discrimination that is not required because of the progress that has been made in the antidiscrimination area. I agree that to some extent, affirmative action has at least tried to address the issues of ethnicity and race based on the ethnic composition of modern population (Selden, 2006). In sum, from the article, it can

A Black Cloud Essay Example for Free

A Black Cloud Essay The heart is an organ of fire, filled with intense love and intense hatred. Yet it is the hatred which entwines the lives of people causing them to display acts of violence and cruelty. Hatred is displayed through forms which include: prejudices towards large groups of people, crimes of hatred being committed, and indirect hatred which involves hurting others as an act of rebellion against someone or something. Throughout history, the world has been afflicted by the prejudices of powerful leaders who in turn took their animosity out on large masses of people. Adolf Hitler, the leader of Germany during World War II, is a prime example of this. He took his hatred out on the Jews, forcing them into concentration camps. Another group that is a target of hatred are the blacks. The Ku Klux Klan, otherwise known as the KKK, possess intense hatred for the black race. Their hatred is demonstrated by marching in white cloaks in protest and also by burning the churches and neighborhoods of blacks. Just as Hitler and the KKK express hatred, the Skinheads are yet another group which have strong prejudices. See more: Basic Economic problem of Scarcity Essay These so called Skinheads abominate most races other than their own. White hooded silhouettes, German Soldiers echoing Hail Hitler! , and the infamous Skinheads are harsh reminders of hatred which exists throughout the world. In the same way that prejudices of large groups of people are expressed, hate crimes are committed to demonstrate acts of hatred. Most recent are the burning of black churches across the South. A crime of this sort shows hatred against the black race. Sometimes leading to manslaughter, racial tensions are abundant. Furthermore, the burning of crosses or flags are offensive crimes that are committed to show a persons hatred for religion or the government. Spousal abuse, child abuse, homicide, and wars between races can only be acts of one thing: hatred. Newspapers are filled with page after page of stories revealing the immense amount of hatred that our society possesses. Although crimes are committed to show hatred directly, there are other crimes which can also show hatred but in ways that are indirect. Think back to the Oklahoma City Bombing; it is a perfect example of indirect hatred. This is true because the motive of the bombers was to attempt to take revenge on the government, but instead the bombers took the lives of innocent people who were in the building. Moreover, the Empire State Building gunmans plan was to take revenge on his bitter enemies, who he blamed for making Palestinians homeless , according to a handwritten note he carried. Instead, one tourist was killed and five others were hospitalized in this attempt. Innocent bystanders, who are at the wrong place at the wrong time, are the ones who suffer most from acts of indirect hatred. A black cloud stifles society, suffocating the lives of people, causing pain and suffering. The eyes of mankind are blinded: unable to see passed prejudices. Generations carry the preconceptions of the ones before. Society needs to see the light and learn to accept.

Unemployment And Its Effect Essay Example for Free

Unemployment And Its Effect Essay Unemployment has always been considered the single most serious individual, social and economic problem since the emergence of industrialization and specialization of work. The ability to work and earn is considered as one of the fundamental cornerstone of any functional society. However, when people who can work are deprived of it and are forced to waste their otherwise productive time that could have invested in their own material l prosperity while adding value to the nation. It has also various personal, moral and ethical dimensions associated with it that plays pivotal role in determining the well-being and prosperity of individuals, family, society and ultimately the entire nation. The combined effect of all of these factors is that unemployment has become the most studied phenomena in modern social and economic studies. The unemployment situation At present the unemployment rate in U. S is close to 4. 4 percent of the working population, which implies that a staggering 6. 7 million people stand unemployed (Employment situation summary, 2007). The social and economic effect of such large number of people being jobless is worrisome for the overall health of the economy. It implies that millions of working hour wasted every day, aggregate loss of hundreds of millions of dollars per day, greater stress on families and individuals, rise in poverty, unequal distribution of wealth, decline in health standards of the unemployed people and their dependents, rise in criminal activities and drug dependency, suicide related instances and increased stress on government social security programs which further translates in constraints on economy and society. Persistent situation of unemployment causes people to loose their self-esteem, position and status in society, pushing them to social margins where they become depressed, alienated and feel redundant. Unemployment also decreases the working potential of unemployed people, so that when they try to return to mainstream their capacity to work and perform is substantially reduced and their skill set lost or obsolete. Types of Unemployment There are following major types of unemployment seen in the labor market (Krugman, 1994). 1. Frictional unemployment- The period of unemployment when people are switching from one job to another. 2. Cyclical unemployment – When the demand of labor in market periodically shrinks and grows due to recession in business 3. Structural unemployment- This type of unemployment is caused when the profile of job seeker and job are mismatched causing stress for the employee and the employer 4. Technological unemployment Perhaps the oldest kind of unemployment which happens due to people being replaced by new machines and technologies 5. Classical unemployment When market undervalues the labor. 6. Seasonal unemployment – Unemployment in those job sectors that are seasonal such as tourism, construction, and sports and game professions. Issue of high and low unemployment Although persistent and high level of unemployment is debilitating, a certain level of low unemployment is considered good for economic growth as it creates incentives for economic growth and contribute to long-term stability and security in job market (Bernstein, Mishel, Schmitt, 2001). Low unemployment rate is an strong indicator of prospering economic conditions and give greater bargaining power in the hands of workers, where they are able to negotiate with employers on better pay-scales and facilities. On the other hand, high rates of unemployment are typically result of economic recession. The business is not in state to supply adequate number of jobs, and workers are put at serious disadvantage in terms of selection of jobs, wages, and security, creating a situation of continuous strain. Bernstien. J, Mishel L. Schmitt, J. . 2001. The State of Working America, 2000-2001: ILR Press: Ithaca, NY. Employment situation summary. 2007. Bureau of Labor Statistics. United States Department of Labor. Retrieved from web on 17. 04. 2007. http://www. bls. gov/news. release/empsit. nr0. htm Krugman, P. 1994. Past and Prospective Causes of High Unemployment. Retrieved from web on 17. 04. 2007. http://www. kc. frb. org/PUBLICAT/ECONREV/PDF/4q94krug. pdf

Hybrid Pi Ce Transistor Model

Hybrid Pi Ce Transistor Model The hybrid-pi model is a popular circuit model used for analyzing the small signal behavior of bipolar junction and field effect transistors. The model can be quite accurate for low-frequency circuits and can easily be adapted for higher frequency circuits with the addition of appropriate inter-electrode capacitances and other parasitic elements. Bipolar junction (BJT) parameters The hybrid-pi model is a linearized two-port network approximation to the BJT using the small-signal base-emitter voltage vbe and collector-emitter voltage vce as independent variables, and the small-signal base current ib and collector current ic as dependent variables. Figure 1: Simplified, low-frequency hybrid-pi BJT model. A basic, low-frequency hybrid-pi model for the bipolar transistor is shown in figure 1. The various parameters are as follows. is the transconductance in siemens, evaluated in a simple mode where: is the quiescent collector current (also called the collector bias or DC collector current) is the thermal voltage, calculated from Boltzmanns constant k, the charge of an electron q, and the transistor temperature in kelvins, T. At 300 K (approximately room temperature) VT is about 26 mV (Google calculator). in ohms where: is the current gain at low frequencies (commonly called hFE). Here IB is the Q-point base current. This is a parameter specific to each transistor, and can be found on a datasheet; ÃŽÂ ² is a function of the choice of collector current. is the output resistance due to the Early effect (VA is the Early voltage). Related terms The reciprocal of the output resistance is named the output conductance . The reciprocal of gm is called the intrinsic resistance . MOSFET parameters Figure 2: Simplified, low-frequency hybrid-piMOSFET model. A basic, low-frequency hybrid-pi model for the MOSFET is shown in figure 2. The various parameters are as follows. is the transconductance in siemens, evaluated in the Shichman-Hodges model in terms of the Q-point drain current ID by (see Jaeger and Blalock): , where: ID is the quiescent drain current (also called the drain bias or DC drain current) Vth = threshold voltage and VGS = gate-to-source voltage. The combination: often is called the overdrive voltage. is the output resistance due to channel length modulation, calculated using the Shichman-Hodges model as , using the approximation for the channel length modulation parameter ÃŽÂ » . Here VE is a technology-related parameter (about 4 V/ÃŽÂ ¼m for the 65 nm technology node) and L is the length of the source-to-drain separation. The reciprocal of the output resistance is named the drain conductance . The COMMON-EMITTER CONFIGURATION (CE) is the most frequently used configuration in practical amplifier circuits, since it provides good voltage, current, and power gain. The input to the CE is applied to the base-emitter circuit and the output is taken from the collector-emitter circuit, making the emitter the element common to both input and output. The CE is set apart from the other configurations, because it is the only configuration that provides a phase reversal between input and output signals High -Frequency -pi CE transistor model The Hybrid-Pi model is a fairly accurate description of the BJT small-signal response up to GHz range. Since the common emitter circuit is considered the most important practical configuration , we seek a CE model suitable for high frequencies. Hybrid -pi or Giacoletto common emitter transistor model shown below. This circuit is quite simple and analysis of circuit using this model are not difficult and give result which are in excellent agreement with experiment at all frequencies for which the transistor gives reasonable amplification. Furthermore , the resisitive components in this circuit may be derived from the low frequencies H-parameters. All parameters (resistances and capacitances) in the model are assumed frequency invariant. Parameters may be vary with the quiescent operating point , but under given bias conditions they are reasonably constant for small signal variations. For high frequency analysis the transistor is replaced this high frequency hybrid PI-model and voltage gain and current gain , input impedances etc are determined. To find current gain Apply current divider rule to the output circuit To find input resistance Applying KVL to input circuit Vs = hie ib + hre vce Vs = ib hie + hre iL RL from equation (1) Vs = ib hie + hre Ai ib RL ( iL =Ai ib ) Substituting in equation (2) Ri = hie + hre Ai RL To find voltage gain Av = since To find output resistance Replace RL by a voltage source. Replace independent sources by internal impedance of the source Applying KC L to the output circuit. iC = hfe ib + i1 iC = hfe ib + vce hoe(4) Applying KVL to input circuit ( hie ib + hre vce) =0 substituting for ib in equation (4) substituting in equation (3) To find output resistance with RL RO1 = RO||RL Since RL is in parallel with the voltage source, total output resistance is the parallel combination of RL and RO Numerical problems Question A common emitter amplifier has the following h- parameters. hie =1KÃŽÂ ©, hre = 10-4, hfe =100, hoe = 12 µmho. Find current gain, Voltage gain, Ri, Ro, power gain. Take RL = 2KÃŽÂ ©. Also find output power take vS = 500 mV ( rms). HYBRID-p EQUIVALENT CIRCUIT To obtain Hybrid-p Equivalent circuit Consider a PNP transistor as shown above. The emitter current IE is divided in to base current IB and a component aIE of the collector current. This division of current takes place in the entire base layer at infinite number of points. For mathematical convenience, it is assumed that the division of current takes place at an imaginary terminal B1. rb1e: It is the resistance of forward biased base to emitter junction and it is the resistance offered to the flow of the current IE. rb1c: It is the resistance of reverse biased collector to base junction. The flow of current in this resistance represents the reverse saturation current Ico due to flow of minority charge carriers. rbb1: It is the resistance of the base layer for the flow of the current IB. This is called base spreading resistance because the division of emitter current is spread across the entire region. aIE: This is the current in the collector due to transistor action. When charge carriers reach the base layer from emitter, the potential gradient at the collector junction will result in the movement of the charge carriers in to the collector. This forms the current. aIE depends on the emitter current IE which inturn depends upon the voltage across base to emitter junction. Therefore, the voltage VB1E controls aIE. VB1E is the independent variable. This depends on charge carrier concentration and temperature. cb1e and cb1c: This is the stray capacitance across the two P-N junction. The reactance of the capacitor is very high at mid-frequency. Hence approximately, capacitors are replaced by open circuit (not considered). But for high frequency, the reactance becomes finite. Hence considered in the analysis. All the above terms are called Hybrid-p parameters. These parameters can be represented by the following circuit and it is called Hybrid-p equivalent circuit or Giacollette equivalent circuit. gm vb1e is the component of collector current(aie) expressed as a function of independent variable vb1e. gm is the Transconductance of the transistor. This represents ability of the transistor in transforming the input voltage vb1e in to output current.rce: rce is the internal resistance of the current source. To find Hybrid-p parameters Hybrid -p equivalent circuit Let the output terminals be short circuited . Considering mid- frequency, reactance of all capacitors becomes infinite. Therefore, all capacitors can be replaced by open circuit. rb1c is the resistance of reverse biased collector junction whose value is very high. Therefore it can be approximated to open circuit. rce is short circuited, becomes redundant. Hence can be removed To find gm where ΆIC and ΆVB1E are the changes in the currents and voltages around quiescent condition. We know that IC = aIE + ICO Since ICO is very small and a is very close to unity, Differentiating with respect to VB1E If t = 27oC (3) substituting in (1) In general In the above equation, IC represents the dc collector current or quiescent current. Its value can be found graphically by drawing the dc load line, locating the Q point on the load line and then measuring IC. OR if know the biasing arrangement of the transistor, then the circuit can be solved using biasing technique and then IC can be calculated. To find rb1e From the two port network theory, we know that vse = ib hie + hre vce (4) ic = ibhfe + hoe vce (5) OR From equation -(5) In the hybrid p equation circuit, VCE is already 0. Therefore obtain the ratio From hybrid p equation circuit and equate it to equation (6). Equating to equation (6). hfe =gm rb1e To find rbb1 From equation (4) From the hybrid-p equivalent circuit, applying KVL to input circuit. Vs = ib(rbb1 + rb1e) To find rb1c Rewriting the hybrid p equivalent circuit by neglecting all capacitances( open circuit) From equation (4) Taking ib = 0 in the hybrid-p equivalent circuit, since there is no voltage drop across rbb1, vs = vb1e. Substituting in equation (8). From the hybrid -p equivalent circuit. Applying voltage divider rule to circuit(2). rb1e is the resistance of the forward biased junction and rb1c is the resistance of the reverse biased junction. Therefore rb1e can be neglected in the denominator. To find rce From equation-(5) Applying KCL at the output terminal ic = i1 + gm vb1e + i2 substituting in the above equation Since rb1e To find Cb1C Cb1C is the junction capacitance of reverse biased collector to base junction. When a PN junction is reverse biased, the width of the depletion layer increases and capacitance decreases. Therefore Cb1C is very low of the order of few pico farads. To find Cb1e This is the capacitance of forward biased PN junction. When a PN junction is forward biased, width of the depletion layer decreases and capacitance increases. Cb1e + Cb1C = Where fT is called the transition frequency. fT = hfe fb fb is called upper cutoff frequency. fb = Numrical Problem: A transistor amplifier is operating with a dc condition of (10V,10mA). The operating temperature is 300C. The H-parameters of the transistor are hie =1Ko, hre =2.5X10-4, hfe=100, hoe=25X10-5mho. Calculate hybrid-p parameters given that CC=3PF. Take fT=1MHz. Solution References and notes ^ R.C. Jaeger and T.N. Blalock (2004). Microelectronic Circuit Design (Second Edition ed.). New York: McGraw-Hill. pp. Section 13.5, esp. Eqs. 13.19. ISBN 0-07-232099-0. ^ R.C. Jaeger and T.N. Blalock. Eq. 5.45 pp. 242 and Eq. 13.25 p. 682. ISBN 0-07-232099-0. ^ R.C. Jaeger and T.N. Blalock. Eq. 4.20 pp. 155 and Eq. 13.74 p. 702. ISBN 0-07-232099-0. ^ a b W. M. C. Sansen (2006). Analog Design Essentials. DordrechtÃŽÂ ¼: Springer. p. 0124, p. 13. ISBN 0-387-25746-2. G.B GUPTA Millman halkias

Hidden Meaning in Laura Esquivel’s Like Water for Chocolate Essay

Hidden Meaning in Laura Esquivel’s Like Water for Chocolate   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚     Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Laura Esquivel’s novel, Like Water for Chocolate, is a contemporary novel based on romance, recipes and home remedies. Very little criticism has been done on the novel. Of the few essays that are written on this work, the majority of them consist of feminist critique. This novel would be most easily approached from a feminist view because of the intricate relationships between women. However, relationships between women are only one of the many elements touched upon in the novel. Like Water for Chocolate is a novel that uses recipes as a crypt for many important themes in the novel. Jaques Derrida defines crypt as something that, "disguise[s] the act of hiding and to hide the disguise: the crypt hides as it holds" (Derrida 14). The recipes are more than just formulas, they hold, concealed within them, memories. These crypts are revealed through food and the process of food production. Esquivel has personal ties with food and feels that the prod uction of food creates a center of the household. Tita, being the person most closely associated with food preparation in the novel, becomes the primary focus in the structure of her family. The crypts that Esquivel uses are opened throughout the novel in a variety of ways. Tita is constantly struggling against her mother, tradition and inevitably her own destiny. Along the way many aspects of her trials are revealed in her cooking. Eventually, Tita is able to free herself from the emotional chains that her mother has bound her. In the end her destiny is revealed, which in return sets her free from her struggles. Esquivel begins each chapter of the novel with a different recipe. The various recipe... ...rodic Consumption of Popular Romance Myths in Como Agua Para Chocolate." Latin American Literary Review. 24.48 (1996): 56-66. Esquivel, Laura. Like Water for Chocolate. Trans. Carol Christensen and Thomas Christensen. New York: Doubleday, 1992. Ibsen, Kristine. "On Recipes, Reading and Revolution: Postboon Parody in Como Agua Para Chocolate." Hispanic Review. 25 (1996): 133-146. Januzzi, Marisa. Laura Esquivel. "Like Water for Chocolate: A Novel in Monthly Installments, with Recipes, Romances and Home Remedies." Review of Contemporary Fiction. 13 (1993): 246-246. Loewenstein, Claudia. "Revolucion interior al exterior: An Interview with Laura Esquivel." Southwest Review. 79.4 (1994): 592-607. Valdez, Maria Elena. "Verbal and Visual Representation of Women: Como Agua Para Chocolate/Like Water for Chocolate." World Literature Today. 69.1 (1995):78-82.

Greek mythology :: essays research papers

The Mycenaeans of the ancient Greeks explained the origin of the universe using Greek gods. The gods were the makers of the universe and watched over mortals.   Ã‚  Ã‚  Ã‚  Ã‚  The Greeks explained that in the beginning there was emptiness, and the emptiness was called Chaos. From Chaos came the first three immortals: Gaea, who was the earth mother; Tartarus, who ruled the Underworld; and Eros who was the god of love.   Ã‚  Ã‚  Ã‚  Ã‚  Gaea gave birth to Uranus without a partner. Gaea then married her son and she bore him three children called the Hundred-handed giants. The Hundred-handed giants each had fifty heads and one hundred incredibly strong arms. These would be the most frightening creatures that Gaea had. Uranus was afraid of their potential power so he confined them to the Underworld. The Hundred-handed giants became mad with their father and hated them with all their might.   Ã‚  Ã‚  Ã‚  Ã‚  Next Gaea had three Cyclopes. Each Cyclopes had one wheel shaped eye in the middle of their forehead. The Cyclopes were very strong and very skillful. Therefore they became the first immortal craftsmen. Again, Uranus became jealous of their skill and potential power, so he bound them with the strongest chains and threw them into their mother. After nine days and nights, they reached the Underworld. They were condemned there along with their brothers, the Hundred-handed giants. They, in turn, also grew to hate their father.   Ã‚  Ã‚  Ã‚  Ã‚  Gaea then bore the first generation of immortal gods. Later, Uranus named them Titans (â€Å"Stretchers†). He named them stretchers because they had stretched their power and had overthrown his rule. Twelve of the thirteen Titans either controlled the major functions of the universe or had children that did.   Ã‚  Ã‚  Ã‚  Ã‚   Cronus was one of Gaea’s children, a Titan. He killed his father for condemning his children. Before Uranus died he prophesied that â€Å" You murder me know, and steal my throne-but one of your own sons will dethrone you, for crime begets crime.† Cronus was very careful from then on.   Ã‚  Ã‚  Ã‚  Ã‚  Cronus’s wife, Rhea, goddess of earth, bore cronus children and one by one he swallowed each and every one of them. First, three daughters-Hestia, Demeter, and Hera; then two sons-Hades and Poseidon. Rhea was very furious with Cronus. She was determined to make sure that Cronus didn’t eat their next child which she was sure was going to be a son. When the time came, she snuck off down the slope of Olympus to a dark place to have her baby.

Comedy Newspaper Aricle about Smoking -- Spoof Joke Anti Smoking

A Smoking Spoof The Daily Smoker Funded by the Institute for Smoking and Hacking and by the makers of FLEM: "Stay happy while we get rid of the nasty." The Smoker's Perspective Nicotine: The Supreme Drug for Supreme Health Staff Writer: Jo "The Butthead" Khamel For many years, anti-smoking groups, such as the American Cancer Association, and government agencies, like the Food and Drug Administration, have confused and mislead the public by incorrectly concluding that smoking is bad and unhealthy. Nonsense! In fact, there are several articles that would support nicotine as a drug that promotes perfect health. For instance, and article by Doug Levy of the USA Today discusses nicotine's power of promoting alertness. Nicotine speeds up the communication between brain cells and stimulates areas of the brain tied to alertness and memory. Mr. Levy also writes about nicotine's relationship toward Parkinson's disease. Levy also writes that cigarette smoking can lower the risk of Parkinson's disease. Researchers claim that something in smoke lowers the levels of an enzyme known as MAO B. Smokers have about 40% less MAO B than nonsmokers or ex-smokers, according to Joanna Fowler of the Brookhaven National Lab in Upton, NY. Because reduced levels of MAO B result in greater amounts of dopamine, smokers may have a lower risk of Parkinson's, a nerve disease aggravated by dopamine shortages. In another article by the Associated Press in the USA Today, researchers suggested that cigarette smoking sharpens short-term learning and memory among young people. Researchers at the University of California, San Diego, tested young smokers and nonsmokers at a word game that required rapid memory and quick recall. The 12 electrodes attac... ...JR Renolds, Inc., and The Council for Smoking and Hacking Research. References Begley, S. "Memories Are Made of...." Newsweek. November 4, 2007. 68. Associated Press. "Research suggests smoking sharpens thinking." USA Today. November 19, 2007. Levy, D. "Nicotine's power on brain tied to smoker's alertness." USA Today, August 8, 2007. ......"Smoking, lower Parkinson's risk linked." USA Today. August 8, 2007. Disclaimer: The odd names (FLEM, Flip Moris, JR Renolds, Institute for Smoking and Hacking) are not real in any form. For certain purposes, those odd names are meant to be comical and should not be affiliated with actual tobacco institutions and companies. However, the cited references in the essay are reported facts found in newspapers and magazine articles. Jo "The Butthead" Khamel is not a real person. If it is, then the use was unintentional.

Record Retrieval System Essay

Chapter 1 The Problem and Its Scope Introduction Technology plays an important role in retrieving patient records in the lives of every patient, an office staff and a medical hospital because of the big boom of technology across the globe it enhanced the services of health institutions. Big, small hospitals or even clinics use the advantage of technology that gave ease to every transaction made especially on important document stored and released for patients. Every record is important just like birth certificates that every parent will retrieve from a hospital for future use of their children, a medical record or laboratory results needed by doctors for medical purposes. For the past decades, medical hospitals all over the world are using a paper system in the retrieval of patient’s records. Patient Record Retrieval is the process of getting the patient records back from a repository or a place where records are stored. Such as, an outpatient record is stored in the deck of folders, envelopes mostly sorted by family names, and there are hundreds or thousands in one of the corner of the office. Patients come and will retrieve records if needed anytime and does not have the assurance that a record will be able to retrieve in a short period of time. Medical centers in the country, it maybe prestigious or not, has also encountered the same problems as other countries encountered, scattered records are everywhere and occupied the whole space of the office, adds manpower for processing transaction and waste of office supplies and most especially retrieval services are slow. Government hospitals have many patients most especially poor people from rural areas and retrieving patient records in the hospital is the common problem. Negros Oriental Provincial Hospital is a government health agency intended for the poor and one of its services is to retrieve records of patients. One problem that clients lost their patience is because of turtle-like services of the agency in retrieving records. Factors that the hospital has a slow service in retrieving patient records and it is because records are very hard to locate that consumes couple of minutes in retrieving for a specific record being requested. In addition to the problems of Negros Oriental Provincial Records Department is that the paper  system consumes the office space, waste of paper materials and additional personnel for locating records. Thus, with all problems encountered by Negros Oriental Provincial Hospital Records Department in retrieving records, the researchers came up a solution to have a new computerized patient records retrieval system. Review of Related Literature, Studies and Systems Review of Related Literature Patients record a collection of documents that provides an account of each episode in which a patient visited or sought treatment and received care or a referral for care from a health care facility. The record is confidential and is usually held by the facility, and the information in it is released only to the patient or with the patient’s written permission. A problem-oriented medical record also contains a master problem list. The patient record is often a collection of papers held in a folder, but it may be computerized. Retrieval of medical record has been a significant means of communication between the Provider, Payer and Legal community. For decades it has been an extremely manual driven, paper driven process and time consuming process. With the advent of technology, issue of regulatory guidelines of PHI security and confidentiality such as Privacy Rules or HIPAA, Medical Record Retrieval and Release of Information is an industry which has undergone and is going through a lot of transformation. Earlier being done by the organizations themselves, this service is now being outsourced to specialists with the end goal to reduce retrieval time by process automation, reduce paper usage, and abide by the stringent HIPAA and Privacy rules. Retrieving medical records isn’t overly difficult, but it is a process with specific requirements. For example, medical records aren’t always stored at a physician’s office. First, the medical provider needs to be contacted and asked about where the medical records request forms should be sent. Once the correct mailing address has been obtained, the record request forms as well as a HIPAA authorization form and a check for copy charges needs to be sent. In a perfect world, the provider would receive the request and fill it right away. However, it’s not unusual for a request to sit in a pile on some clerk’s desk making follow-up calls an absolute necessity. Lehnart et al. defines a patient record management system as a system that stores demographic, and medical information from ancillary services such as registration, lab, radiology, pathology, pharmacy, consultation and transcription.They state that a record management system is not simply automated updates of paper based charts, but rather a dynamic system used to help health care workers make better informed dragonesses. According to Gaillour et al. a record management system is only effective at achieving the goals of increasing quality of care and lowering costs if the organization re†designs it’s current workflow and practices. Hence a very user† friendly system needs to be created to mitigate the risk of user dissatisfaction towards the new system. Fromberg et al. claim the clinical benefits to such a system includes easier, more rapid access to patient data charts; Improved clinical decision making and disease management; More educated patients about their own ailments; An increase in time to spend consulting with patients; An increased perception of patient care and theoretically a better working environment. All these benefits overall translate to better patient care. More benefits include a more efficient workflow, as duplicate tasks would not be performed as the need to re gather information would be eliminated as all data pertaining to the patient is readily available at all times. Time taken to execute administration functions, such as capturing patient demographics, drop dramatically. According to Dassenko and Slowinski, an average of up to 15 minutes was saved per patient on the patients first visit and further 20 minutes on each subsequent visit as a result of implementing a computer based patient record. Fischer and Bloude (1999) states findings that the retrieval of paper records was time consuming and finding the required information amongst many documents for certain patients could be a lengthy process. Wellen et al (1998) emphasized the advantage of EPR because it enables information be Time is saved by EPR in not requiring the whole patient file to be used to find to available quickly about specific requirements perhaps just one piece information. Coiera (2003) EPR added that information instantaneously drugs though many documents. with EPR instead of requiring a search such as allergies to particular could be obtained information strategy states that in the EPR system the patient records should be secure, accurate, and legible. They can be easily accessed by authorized staff and in addition to use for individual patients can be incorporated in research. Data can also be available for the improvement of quality. Bush (2002)recommended introducing and using an appropriate EPR system since it helps to reduce costs and ongoing expenses in providing multiple users access to faster. Distance is information, data protection and backups. An EPR backup system is more economical than the manual system since it saves space, time to locate and access information and maintenance costs. Abdellhak et al (1996) and Young (2000) state that physical control of a file are not always available, EPR data can be accessed at any provides adequate security. However, sometimes as many as 30% of which is not possible with paper records. Englebardt and Nelson (2002) and Reynolds (2003) agree that an increasing number of paper records approved workstation are authorized difficult more Robert(2002) one the information for the right reason. Especially Friedman (2005) considered security for confidentiality must be ensured this is and relevant with EPR systems even if it makes authorized access access patient record especially in emergency cases. Kirshna and authorized person. Where as a paper based system is available to only one person at a time to point out that an EPR system can  be used simultaneously by more than Amatayku (2004) drew attention to access policy which needs not only a ban sharing passwords but also a reminder of the possible legal consequences. Schmitz (1979 p.74) described an early EPR system at a time when â€Å"there is as yet no such thing as a fully electronic medical record†. The benefits were then seen to be â€Å"timeliness, accuracy, completeness and availability† resulting from†having physicians interact directly with an electronic management information system† (Schmitz 1979, p.75). He seems to have been one of the pioneers in anticipating the potential of EPR, and the benefits from professional input. Kovner (1990), who considered the use of electronic records for patient history and current treatments. The availability of computer systems from 1990 onwards further helped to prove the arguments raised by the two authors above. This availability changed medical record keeping to electronic methods, which were beneficial in many ways. methods of recording have reduced the size of records despite the fact that they contain very much more information. By using computers, doctors can easily access information from more than one source. The organization of records for ease of access is essential for efficiency and the importance of the service is recognized by supervisors in charge. Good organization and management requires good leadership to ensure efficiency and co-operation and a constant improvement performance. In addition to the above observations, Meijden et al (2000) measured the attitudes towards electronic patient record among physicians and nurses. The researchers noted that the experienced physicians and nurses were move positive, whereas the inexperienced ones found EPR to be more time consuming for data entry and retrieval, and they were concerned about their familiarity with computers and the need for training. This study implies that one has to be experienced in making the optimum use of EPR systems. Furthermore, an EPR system proves to have more effect on improving quality of patient care. Bickford (1995)noted the in as a restriction potential that EPR systems have for improving patient health costs, adding satisfaction for providers, researchers and administrators. Dick and Steen (1991) argued that patient records should include more information than just treatment details for as proposed by earlier researchers such as Kovner (1990), example, guiding problem solving, decision analysis, reminders, and risk assessment ,an do the relevant details(Dick & Steen1991,p.37). The system could prompt staff about additional considerations not available in paper records. The system would be accessible at all times. Similarly, a report by the Institute of Medicine (IOM 1997) helped to argue further that an electronic patient record is to be as the one that is specifically designed to support users through of complete and accurate data, practitioner reminders and alerts, clinical understood availability decision support systems, links to bodies of medical knowledge and other aids. Novak(2005) considered EPRs as time saving which can be life saving, transfers to cost other physicians history, effective whilst maintaining confidentiality and, making easy and immediate. However A personal EPR can contain a total medical complicated. EPR systems have to a greater extent improved patients records and facilitated  the selection of the most appropriate treatment. Amongst these advantages, Burton et al with the patient’s input can be created when records are retrieved much Lane & Hayward (1999) investigated the value of electronic patient records make adequate and legible records has been reduced to take only a few minutes per patient, when physicians’ time is tightly scheduled. However, Soper (2000) observed that more time to see a patient, together commented that the time taken to. Furthermore, the above author noted that accessibility of record sat a made possible. Electronic records are more legible and can resolve the problem of misplaced documents and the opportunity to show parents the records of their children if required. For  GPs and found them to be considerable, but there were doubts about the system on a larger. Furthermore, (Atkinson 1997; British Medical Association 2002). The training of users on EPR scale in hospital use. Mansoor (2002) Training the users in manipulating EPR systems has proved to be easier them to familiarize themselves with other aspects of computer supports the observation in that physicians use systems motivates applications. Computers for administrative purposes as well as EPR systems, and are keen to acquire computer skills and knowledge to enhance their clinical practice. They learn how to access computer based information and to how to make the best use of such resources. Svenningsen (2003) found the advantages of EPR included no loss of records, ease of access for all medical staff, some reduction in professionals. The same was the case for Smith, (2003) who considered good medication errors, better documentation, and more co-ordination between leadership and supportive staff were essential for a planning, strong successful EPR system. Benefits include accurate medication lists, legible notes and physician. Having experienced EPR he would never revert to paper records. Seems to summarize the general point of view of those who have experienced prescriptions. This the change from manual to electronic systems. Amatayakul (2005) emphasized the value of EPRs providing reminders to alert hospital staff to particular problems which may arise, and improve decision making, in addition to reducing errors. At the same time much information is still being handwritten in many hospitals and the electronic records do not necessarily include information which would assist decision making. Also it was helpful to have systems which work similarly in different places e.g. surgeries, clinics and hospitals. The benefits of EPR as outlined by Ginneken (2002, p. 115) included Flexibility in content and use, integration and adaptability to change. Once consensus is reached on terminology, architecture, and legislation, the EPR  will become as established as the Hippocratic Oath record has been for centuries†.It seems from the literature that benefits have been obvious in all the countries that have adopted the system, and even those who originally had difficulties in making the changes now express no regrets, because they have experienced the great advantage of electronic systems. There was a reported reluctance to change which needs to be overcome by a good training programme, and some people under-estimate their capabilities to cope with different systems ( Loomis & Ries 2002). As Huston (2004) noted, to such a change would require an agreed standard procedure and provision for the transition period. Faber(2003)draws attention to the fact that several authors new and implement argue that the implementation of EPR can fail if the assumed nature of the medical work being considered does not match the real aspects of that work. Related Studies and System According to the study of abdullah, f. Epr system in hamad medical corporation   Qatar that it greatly resolved the dissatisfaction with the existing manual patient  Record system expressed both doctors and nurses referred to many defects of the  Present manual system which caused irritation and potential exposure to reducing  Patient safety. Such obstacles can affect the improvement of patient care and delay Important treatment. From the findings the major problem of the traditional paper  Based record system appears to be misfiling of records causing difficulties in obtaining   Information quickly in emergencies. Thus the newly EPR system benefited the   Hamad medical in terms of the accuracy,legibility, confidentiality and time saving  in the patient records. Another study from Droma, Fahad et al. in automation of the patient record management sytem in St Francis Hospital Nsambya that Patient record management systems in hospital today necessitate a competent administration when handling patients, generating reports from cashier, patient details which serves as a key factor for the flow of business transactions in St Francis Hospital Nsambya. Unfortunately the current Record management system leads to misplacement of drug details, payment details, and late release of reports and insecurity to records. This research project is aimed at computerizing all the records about patients, staff and drug suppliers. In order to achieve this goal, a thorough System Study and investigation was carried out and data was collected and analyzed about the current system using document and data flow diagrams. The concept of report production has been computerized hence, no more delay in report generation to the hospital manager. Errors made on hand held calculators are dealt out completely The method used to develop the system include iterative waterfall model approach, dataflow, logical and entity relationship diagram were used to design the system and finally the language used were MySql, php, HTML, CSS and JavaScript. Atkinson (1997), whilst seeing the benefits of EPR systems, also that they could change clinical practice, and that there could be problems of controlling access to them. His research also reported anxieties that were expressed regarding the possibility of computer failure. The argument shows that the electronic record system has a â€Å"back up† scheme for computer failure patient which automatically prevents information from being lost. In addition, even if the benefits of EPR are recognized,found that the time taken to learn procedures was an obstacle to their use. A software engineering consultant, Sam Simple was hired to design a Computerized Patient Record system for a hospital. Each patient’s record in the database consists of a patient’s name, address, age, phone number, next of kin, name of parents, phone number, birth date and place, social security number, occupation, marital status, religion, military service, treatment history, family background, lifestyle information such as drug history and sexual  preferences, diagnostic and testing information, and insurance information. At the initial meeting held to discuss the project requirements, a hospital representative indicated to Simple that the hospital had conducted research on CPR systems prior to hiring him. Based on the report produced from the hospital’s research, it considered that user authentication to verify users’ ID and password at login was sufficient for their system, as far as the system security was concerned. However, Simple learned from a study that 85 percent of the passwords on a typical computer system were guessable. According to Dr. Marie Sy, CHITS, an electronic medical record (EMR) specifically designed for the community health centers in the Philippines, was developed through a collaborative and participative process involving health workers and the Information and Communication Technology (ICT) community, using the primary health care approach and guided by the open source philosophy. â€Å"The development of CHITS that the paper record retrieval time was decreased from 2.41 minutes to less than 5 seconds,† has resulted in increased efficiency of health workers, allowing them to spend more time for patient care, improved data quality; streamlin ed records management; and data-guided decision-making, both operationally and strategically,† Dr. Sy added. The development of EPR in most countries has been rapid in recent years with some differences to meet local requirements. Beaumont (1999) noted the advantage of electronic records in the UK, including simultaneous access from multiple locations, legibility, ease of exchange of data, and confidentiality. He compares the advantages with those of paper records which are: easily transported; easy to read; require no training and are never â€Å"out of order†. According to Beaumont’s personal experience, electronic records are an improvement on medical handwriting which is often illegible. In addition, he noted a need for training in the details of categories which the manual records should contain, just as computers may be â€Å"down†, so misfiled patient records can be equally frustrating. Frolick, (n. d. ) noted that electronic patient records in USA are of great benefit to patients, because they are not subject to loss, illegibility or inaccuracy, and assist in guiding patients’ daily treatment. In addition, the records would be readily available for research and accessible directly on the users’ screen. Furthermore, Madison(1997) reported Dr. Paul King’s opinion that the ability to create, and retrieve  charts quickly was of importance and time saving. The choice of the best EPR in USA for a particular department as important and there should be a wide range of availability of a system for selection. By the immediate accessibility of the right technical information lives could be saved, and this is the most important consideration of all. In addition to aspects such as time saving and quality of care, Kowalsky (2002)observed that, to make the system comprehensive and cost effective was a large task and integrating existing systems was difficult. Much as the EPR systems are suggested to provide the best solutions to improve patient record keeping, several authors have observed a number of challenges to them. For example, Fields & Duncker (2003) mentioned that although EPR systems are planned to be universal in UK by 2008, there were doubts about this. The complexity of the task and the need to complete it rapidly caused concern after previous NHS computerisation problems. There was anxiety and the need to convince staff of the benefits of the system. Bishop (2003) referred to availability, as being able to use the information or the source desired i. e., hardware, software or networks. The same issues were discussed by Singh et al. (2004) who noted that primary care in USA is complex and includes safety problems, with no two providers being alike. EPR imposed on any health system can have unpredictable effects, reducing or increasing safety. To some extent EPR could distract a GP from properly recording observations, but, if used correctly, would greatly assist in providing immediate and accurate information. In practice it is essential that all staff are familiar with EPR systems, if these are to be used effectively, and all aware of hazards and how to avoid them. Similarly, Pizziferri et al (2005) considered one factor which inh ibits the use of EPR in USA was the concern that it may take more time than paper records. A study of 20 physicians’ use of time at primary health centres was recorded, before and after the introduction of EPR, and a decrease in time was noted. This was also the case with dictating notes, reading, and writing; however searching for data was much faster than before. The researchers concluded that EPR took less time than manual records but that there was a need to identify EPR users who had difficulty with the system. Mikkelsen & Aasly (2005) of the neurology department at St Olave’s Hospital, Norway analysed electronic patient records and how the system affected performance, e. g. the ability to access information. Records for a neurological department were of variable accuracy because of lack of precise definitions, and were a potential threat to the safety of the system. Strict procedures are required to ensure accuracy and sufficient relevant information. The Steiermà ¤rkischeKrankenanstaltenGes.m.b.H. (KAGes), the governing body of the Styrian hospitals. Out of a new MIS, termed OpenMedocs, has been conducted. This system shall simplify the management, the access to and the exchange of health-related patient information. It is a centrally managed system at the headquarter of KAGes in Graz. The core of OpenMedocs is an electronic patient record (EPR) system. All documents concerning patients are stored in this system. Thus, it is possible to receive documents from a patient which have been generated in different hospitals ’at the push of a button’. Since almost all medical information of the hospitals concerning patients is managed in the ERP system, it is possible to avoid various disadvantages of ’traditional documentation’, like multiple medical attendance or local constraints of usage of retrieval possibilities, and user-oriented presentation of data should help, among other things, to speed up and to improve the quality of the medical decision-making process of physicians. Since the roll-out of OpenMedocs, the amount of these patient-related documents increased continuously. Thus, the efï ¬ cient storage and the timely retrieval of documents in the EPR system have gained considerable importance. The Problem Statement of the Problem This present study tries to analyze, design, develop, test and implement a Record Retrieval System for Negros Oriental Provincial Hospital. This study attempts to answer the following questions. 1. What is the current retrieval system used by Negros Oriental Provincial Hospital Records Department in retrieving records? 2. What are the problems encountered by retrieval section employees in the retrieval of patient’s records in NOPH Records Department? 3. What are the requirements needed for the development of the Computerized Retrieval System for Negros Oriental Records Department Retrieval Section? 4. How secure and manageable is the Computerized Retrieval System for NOPH Records Section? 5. How beneficial is the Computerized Retrieval System for  Negros Oriental Provincial Hospital Records Department Retrieval Section. Hypothesis H0: There is an existing problem with the current retrieval system of Negros Oriental Provincial Hospital Records Department. H1: Negros Oriental Hospital Records Department is in need of a new system that will help them improve their service. Technical Background Design Concept Input Data Captured Process Working with records Output Showing results by print outs Storage of the patient records Figure 1 It shows the input, process and output of the patient record retrieval system that in input in order to retrieved such records they need a personal information and the records they want to be retrieved in a system. In Process it is where the records has been process in order they could release the record and in output it is where the records have been already retrieved and ready to release to the authorized person. Design Method Figure 2 Agile Approach The methodology that use in developing the system is agile the researcher use  agile since it promotes adaptive planning, evolutionary development and delivery; time boxed iterative approach and encourages rapid and flexible response to change. A conceptual framework promotes foreseen interactions throughout the development cycle. There are five phases in this methodology the requirements, design, implementation, test and deployment. In requirements phase in which the requirements for the software are gathered and analyzed. This is equivalent to researching and brainstorming what the product requires. Examples can include general features, architecture discussions, workflow discussions and general product discovery. Design phase this is which will have all the requirements defined for the product. Implementation phase during the development, needed to test the code as well as get feedback from the customer on progress. Feedback from the customer can include mockups, front-end designs, and usability. Testing phase bugs and defects are always a constant in the software development process. It is important that there are good quality assurance standards to eliminate general issues. Deployment the software application is finally deployed and live. Once this occurs, a support plan needs to be in place for maintenance and general support on potential future issues. Significance of the Study Negros Oriental Provincial Hospital Record Department.The study will serve as a new instrument for the technological advancement that greatly benefits for Negros Oriental Provincial Hospital. This computerized system is intended to lessen the manpower which will lead to a faster and more accurate record retrieval process which will lead to profitability of the Negros Oriental Provincial Hospital. Employees. The job of the employees will become more accurate and efficient through the use of the proposed system. This leads to less error which saves time and energy on the side of the workers. Employees can also focus on other tasks assigned that will make them more productive. Patients. The service of Negros Oriental Provincial Hospital to the patient will become more convenient. Less time will be consumed during the retrieval process. This will also lessen time for patients to wait for their records to be release. The study is also expected to increase the satisfaction of the patients to the services of the Negros Oriental Provincial Hospital. Researchers This study is a great achievement for the researchers because it  will improve their skills in technical writing. The experiences while doing the research build up their characters and teach them values like creativity, working hard, team building and responsibility and time management. It also builds friendship and camaraderie among the co-researchers. It also gives them an overview of the IT industry and trains them to prepare to the competitive professional field. Questionnaire Directions: Please put a checkmark on the selected choice. Name (Optional):____________________________________ Age:______ Gender:____________ Department:______________________ I. The current system that NOPH are using in retrieving records: 1. What is the present system used by Negros Oriental Provincial Hospital Records Department Retrieval Section? __ Manual Retrieval __ ComputerizedRetrieval If manual, what are the tools or things used in retrieving records or how a single record is retrieved according to its arrangement? _By folders _By logbooks _By envelopes _By family names _By cabinets _By disease/injury Others (Specify):______________________ If computerized, what are the applications used? _ Microsoft Excel _Microsoft Word _Microsoft Access _Others(Specify):_____________________________ II. The problems encountered by employees in retrieving records: 2. What are the problems encountered by Negros Oriental Provincial Hospital Records Department in retrieving records? _Unarranged Documents _Difficulty in finding records _Lost Documents _ Mountainous Stocked Files _Crowded area Others(Specify):______________________________ III. Particular documents that Negros Oriental Hospital retrieve. 3. What are the common documents that hospital clients retrieved mostly? _Birth certificates _Death certificates _Laboratory results Others(Specify):___________________________________ References: Related Literature http://medical-dictionary.thefreedictionary.com/patient+record http://www.wipro.com/industries/healthcare-segments/medical-records-retrieval.aspx http://www.sunbeltreporting.com/sunbelt-blog/bid/45059/Benefits-of-Outsourcing Medical-Records-Retrieval) Fischer, J. S. & Blonde, L., 1999. Impact of an electronic medical record on diabetes practice workflow. Clinical Diabetes, 17(2), 10-12. Wellen, D. et al., 1998. The electronic medical oncology record: misconceptions, barriers, and benefits. Cancer Management, 3 (5), 6-8. Coiera, E., 2003. Guide to health informatics. 2†³d ed. London: Arnold. Bush, J., 2002. Looking for a good electronic medical records system? Family Practice Management, 9(1), 50-51. Lenhart, J. G., Honess, K., Covington, D., and Johnson, K. E. â€Å"An Analysis of Trends, Perceptions, and Use Patterns of Electronic Medical Records Among Family Practice Residency Programs.† Family Medicine, February 2000, 32: 109† 114. Gaillour, F. â€Å"Rethinking the CPR: Is Perfect the Enemy of the Good?† Healthcare Management Technology[serial online], May 1999, 20: 22†25.32 Fromberg, R., and Amatayakul, M. â€Å"CPRI and the Future of Computer†based Patient Records.† Healthcare Financial Management, July 1995, 49: 48 Dassenko, D., and Slowinski, T. â€Å"Using the CPR to Benefit a Business Office.† Healthcare Financial Management, July 1995, 49: 68†70, 72†73 Abdelhak, M., et al., 2001. Health information: management of a strategic resource. 2nd ed. Philadelphia: W. B. Saunders. Englebardt, S. P. & Nelson, R., 2002. Health care informatics an interdisciplinary approach. USA: Mosby. Friedman, B. 2005. Health Records get personal: a technology outlook for consumer access to personal health information. Journal of American Health Information Management Association, 76(1), 42-45. Amatayakul, M., 2004. Electronic health records: a practical guide for professionals and organizations. USA: AHIMA. Schmitz, H. H., 1979. Hospital information systems. London: Aspen Systems Corporation. Kovner, A. R., 1990. Health care delivery in the United States. New York: Springer Publishing Company. Meijdan, M. J. V. et al., 2000. The user in the design process of an EPR. Studies in Health Technology and Informatics. 77,224-228. Bickford, C. J., 1995. The concept model of the electronic health record development of the CPR and CPRs concept models. Toward an electronic patient ecord? 95 Proceedings. 0 ed. IL, USA: Kelvyn Press. Dick, R. S. & Steen, E. B., 1991. The computer-based patient record, an essential technology for health care. Washington D. C.: National Academy Press. Novak, K., 2005. Reducing costs through electronic health records and services. Benefits and Compensation Digest, 42(10), 40. Burton, L. C. et al., 2004. Using electronic health records to help coordinate care. The Milbank Quarterly, 82(3), 457-581. Lane, V. & Hayward, P., 1999. Medical records. The Lancet, 353 (9149), 330. Soper, W., 2002. Why I love my EPR. Family Practice Management Journal, 9(9),1-7. Mansoor, E., 2002. Computer skills among medical learners. Journal of Ayub Medical College (JAMC), 14(3), 13-15. Svenningsen, S., 2003. Electronic patient records and medical practice, reorganization of roles, responsibilities, and risks. PhD thesis, Department of Organization and Industrial Sociology, CBS- Copenhagen Business School. Ginneken, A. M. V., 2002. The computerized patient record: balancing effort and benefit. International Journal of Medical Informatics, 65(2), 97-119. Amatayakul, M., 2005. Are you using an EHR-really? Healthcare Financial Management, 59(11), 126-128. Loomis, G. A. et al., 2002. If electronic medical records are so great, why aren’t family physicians using them? Journal of Family Practice, 51(7), 636-641. Huston, J. L., 2004. The need for mandatory clinical recording standards. Clinical Medicine, 4(3), 255-257. Faber, M. G, 2003. Design and introduction of an electronic patient record: how to involve users? Methods of Information in Medicine, 42(4), 371-375. Related Studies Abdullah, Foziyah., August 2007.Electronic Patient Records System in Hamad Medical Corporation, Qatar: Perspectives and Potential Use. Droma, Fahad. et al.,An automated system for patient record management: a case study of St. Francis Hospital Nsambya. http://dspace3.mak.ac.ug/xmlui/handle/10570/443 Atkinson, C., 1997. A case study on development an electronic patient record in the UK. Requirements Engineering, 2(1), 1-14. Kibbe, David, MD, MBA, and Bard, Mark R., MHA, MBA. (1997). How Safe Are Computerized Patient Records?. Journal of Family Practice Management, May 1997 Vol. 4 No. 5 . Retrieved April 2, 2002 from American Academy of Family PhysiciansWebsite: http://cpmcnet.columbia.edu/dept/dental/Dental_Informatics/AOFC_Course/DI_Clinical/CPR.html http://www.pchrd.dost.gov.ph/index.php/2012-05-23-07-46-36/2012-05-24-00-01-11/5111-electronic-medical-record-system-chits-can-retrieve-patient-record-in-five-seconds-expert Beaumont, R., 1999. The electronic patient/healthcare record (EPRIEHR). 8 `h ed., [accessed 5.4.2004]. Frolick, M. N., [n. d. ]. Using electronic medical records to improve patient care, , [accessed2 6.03.2004]. Madison, D., 1997. Breaking away from paper. Healthcare Informatics, 14(10), 4-6. Kowalsky, C. A., 2002. The computerized patient record. Journal of Medical Education, 3(3), 1-6. Fields, B. & Duncker, E., 2003. The impact of electronic health records on crossprofessional healthcare work,

Mitosis Replication of Eukaryotic Cells

Exercise 13MITOSIS: REPLICATION OF EUKARYOTIC CELLS  ANSWERS TO QUESTIONS1.a. Mitosis and cytokinesis are often referred to collectively as â€Å"cellular division. † Why are they more accurately called cellular replication? The result of mitosis is production of two cells (replicates) identical to the parent cell. The genetic material is replicated rather than divided.b. Does the cell cycle have a beginning and an end? The organization of our study of cellular events indicates a beginning and end, but the events continue to repeat.2.a. If a nucleus has eight chromosomes during interphase, how many chromosomes does it have during metaphase? During metaphase there are 8 double-stranded chromosomes.b. How many does it have after mitosis is complete? After mitosis (and separation of chromatids) there are 16 chromosomes distributed between two nuclei.3.a. Why would we choose an embryonic mass of cells for procedure 13. 3 in which to study the stages of mitosis? embryonic cells a re replicating and growing rapidlyb. Which stage of mitosis most often is associated with the beginning of cytokinesis? usually late anaphase4.a. What region of a root has the most mitotic activity? meristem, just above capb. Why is pinching of the cytoplasm inadequate for cytokinesis in plant cells? Pinching of cytoplasm is not appropriate for rigid cell walls.c. Locate a plant cell in late telophase. What is the volume of the new cells relative to a mature cell? Each cell is smaller (approximately 1/2) than the parent cell.5.a. Why are the combined data from all the class members more meaningful than your results alone? Combined results reduce random error.b. How accurate were your predictions for length of each stage of mitosis? probably not closec. What sources of error can you list for this technique to determine the time elapsed during each stage of mitosis? ariation in onion roots; student's ability to distinguish stages; microscopic cross sections of roots may or may not pas s through the nuclear materialExercise 14MEIOSIS: REDUCTION DIVISION AND GAMETOGENESIS ANSWERS TO QUESTIONS1. a. Why would shuffling genetic material and producing new combinations of characteristics be advantageous to a species? New genetic combinations allow adaptation to changing environments.b. When would it be deleterious? New combinations always produce a percentage of disadvantageous characteristics; the risk of bad gene combinations may be inappropriate in a stable, beneficial environment.2. a.Synapsis occurs after chromosomal DNA has replicated. How many chromatids are involved in crossing-over of a homologous pair of chromosomes?b. Suppose synapsis occurred between two homologous chromosomes, and one had alleles for blue eyes and brown hair and the other had alleles for green eyes and blonde hair. How many different combinations of these alleles would be possible? 4 Remember that crossing-over does not necessarily occur between the loci, so the original combinations may re main intact. 3. a. If a nucleus has eight chromosomes when it begins meiosis, how many chromosomes does it have after telophase I? Telophase II? with two chromatids each; 4 with one chromatid eachb. What are the major differences between the events of meiosis and mitosis? (see table 14. 2)c. What are some minor differences, and why do you consider them minor? minor differences would be those unrelated to chromosomal events4. a. During gametogenesis a sperm cell undergoes considerable structural change. What are the basics of sperm structure and how does it relate to function? The basics include small head with nucleus; an acrosomal process with enzymes to digest the outer membranes of the egg; a head and neck rich in mitochondria; and tail used to propel the cell to the egg.b. What is the advantage of producing sperm in a system of tubes rather than in solid tissue? because sperm cells can be expelled easily from the body through a system of tubesc. What is each strand of a double-s tranded chromosome called? chromatid5. How would retaining extra cytoplasm enhance survival of a developing oocyte? More cytoplasm contains more nutrients to ensure survival.6. a. What are the relative sizes of oocytes in a dormant follicle, a growing follicle, and a Graafian follicle? dormant oocyte 1X, growing oocyte 5X, mature oocyte 25Xb. Are polar bodies visible in your prepared slide of a cat ovary? Why or why not? probably not, because cells in the ovary usually have not finished meiosis I, and polar bodies disintegrate rapidly DNA FINGERPRINTING LABRestriction Digestion of DNA Samples Observation QuestionsDescribe the samples of DNA (physical properties). The DNA samples are clear, colorless liquid samples.Is there any observable difference between the samples of DNA? No. All samples appear similar.Describe the appearance of the restriction endonuclease mix. The restriction enzymes appear to be clear, colorless liquids.Restriction Digestion of DNA Samples Review QuestionsBef ore you incubated your samples, describe any visible signs of change in the contents of the tubes containing the DNA combined with the restriction enzymes. DNA + EcoRI/PstI enzyme mix: No visible change apparent in the tubes.Can you see any evidence to indicate that your samples of DNA were fragmented or altered in any way by the addition of EcoRI/PstI? Explain. No. No visible change apparent in the tubes.In the absence of visible evidence of change, is it still possible that the DNA samples were fragmented? Explain your reasoning. Yes. They may be chemically changed but the changes may not be visible. Enzymes may have cut the DNA.After a 24 hour incubation period, are there any visible clues that the restriction enzymes may have in some way changed the DNA in any of the tubes? Explain your reasoning. No. No visible change apparent in the tubes but the enzymes may have cut the DNA. The reactions are at the molecular level and too small to be seen.Electrophoresis of Your DNA Samples Review QuestionsThe electrophoresis apparatus creates an electrical field [positive and negative ends of the gel]. DNA molecules are negatively charged. To which pole of the electrophoresis field would you expect DNA to migrate (+ or -)? Explain. Positive.What color represents the negative pole? Black.After DNA samples are loaded in wells, they are â€Å"forced† to move through the gel matrix. Which size fragment (large vs small) would you expect to move toward the opposite end of the gel most quickly? Explain. Smaller. There is less resistance to their movement through the gel matrix.Which fragments are expected to travel the shortest distance [remain closest to the well]? Explain. Larger. There is more resistance to their movement through the gel matrix.Lesson 4 Thought QuestionsWhat can you assume is contained within each band? DNA fragments.If this were a fingerprinting gel, then how many kinds (samples) of DNA can you assume were placed in each separate well? One.What wo uld be a logical explanation as to why there is more than one band of DNA for each of the samples? The DNA must have been cut into fragments by restriction enzymes.What probably caused the DNA to become fragmented? The chemical action of the restriction enzymes cutting at specific base sequences.Which of the DNA samples have the same number of restriction sites for the restriction endonuclease used? Write the lane numbers.Lanes 2, 3, and 4 (CS, S1, and S2).Which sample has the smallest DNA fragment? The sample in lane 5 (S3).How many restriction sites were there in lane three? Two sites that cut the sample into two fragments.Which DNA samples appear to have been â€Å"cut† into the same number and size of fragments? Lanes 2 and 4 (CS and S2).Based on your analysis of the photograph, what is your conclusion about the DNA samples in the photograph? Do any of the samples seem to be from the same source. If so which ones? Describe the evidence that supports your conclusion.The DN A samples in lanes 2 and 4 (CS and S2) are from the same individual because they have identical restrictions sites that yield identical fragments.DNA Fingerprinting: OverviewWhat are we trying to determine? Restate the central question. We are trying to determine if samples of DNA that we were provided with are from the same individual or from different individuals.Which of your DNA samples were fragmented? What would your gel look like if the DNA were not fragmented? The number of fragmented samples will vary. They will have one band on the gel if the DNA was not cut.What caused the DNA to become fragmented? The addition of restriction enzymes.What determines where a restriction endonuclease will â€Å"cut† a DNA molecule? A special sequence of bases on the DNA called restriction sites.A restriction endonuclease â€Å"cuts† two DNA molecules at the same location. What can you assume is identical about the molecules at that location? The restriction sites are identical .Do any of your suspect samples appear to have EcoRI or PstI recognition sites at the same location as the DNA from the crime scene? The samples in lanes 2 and 5 match (CS and S3).Based on the above analysis, do any of the suspect samples of DNA seem to be from the same individual as the DNA from the crime scene? Describe the scientific evidence that supports your conclusion. The CS and S3 samples appear to be identical. They both produce similar banding patterns on the gel.Exercise 23 SURVEY OF BACTERIA: KINGDOMS ARCHAEBACTERIA AND BACTERIA ANSWERS TO QUESTIONS1. a. Why is it important that bacteria release nutrients? Nutrients must be released and recycled for use by other living organisms. If nutrients were not released by decomposers, the nutrients would all eventually be locked up in dead, non-decaying tissue.b. What term would best describe heterotrophic bacteria that feed on living tissue? parasitism2. What is the shape and size of each bacterial colony? E. coli—medium rod; B. megaterium—large rod; R. rubrum—small and thin spirillum 3. a. Which type of bacteria is most prevalent in the sample from your teeth? How do you know? probably gram positive because most of the cells are purpleb. Is Bacillus megaterium gram positive or gram negative? gram positive4. a. Where are the bacteria? Are they between cells or inside cells? inside the cellsb. Why is this relationship between a plant and bacterium called mutualism? Both partners benefit from the association.c. How does Rhizobium benefit from this association? nutrients from the hostd. How does the host plant benefit from the association? nitrogen supply from the bacterium5. Based on their appearance, which drugs or chemicals retard the growth of bacteria? depends on the drugs that you use6. Do all cells of a trichome of Oscillatoria appear similar? yes7. a. Do adjacent cells share a common sheath? usuallyb. What do you suppose is the function of the sheath? protectionc. Do clusters of Gloeocapsa represent multicellular organisms? Why or why not?No, the cells are not obligate dependents—they can operate independently; the protoplasms of the cells are not connected; the aggregation of cells can be broken into individual cells without killing the cells.8. a. How is the shape of Merismopedia different from other cyanobacteria you studied in this exercise? Merismopedia is a flat, square colony one cell thick.b. How would a colony attain this shape? equal divisions in two planesExercise 24Survey of Kingdom Protista: The Algae ANSWERS TO QUESTIONS1. a. Is the movement of Chlamydomonas smooth or does it appear jerky? relatively smoothb. Can you see both flagella? You may need to reduce the light intensity to see flagella. probably not, depends on student's microscopec. How does methylcellulose affect movement of Chlamydomonas? slows it downd. How does the stigma help Chlamydomonas survive? detects light2. a. Under what environmental conditions would a zygote not u ndergo meiosis immediately? unfavorable conditionsb. Are spores of Chlamydomonas haploid or diploid? haploidc. Which portions of the life cycle of Chlamydomonas are haploid? all portions from meiosis to syngamyd. Which are diploid? all portions from syngamy to meiosis3. a. Are the filaments of Spirogyra branched? nob. What is the shape of the chloroplasts of Spirogyra? spiralc. Can you see any conjugation tubes? If you can't, examine the prepared slides that demonstrate these structures. probably not seen on live material, but easily seen on prepared materiald. How do you think that Spirogyra reproduces asexually? mitosis and fragmentation4. a. How is Cladophora morphologically similar to Spirogyra. How is it different? They are both filaments, but Cladophora is branched and Spirogyra is unbranched.b. What is the shape of its chloroplasts? broad and dispersed5. a. What is oogamy? ne gamete (sperm) is small and motile, the other gamete (egg) is larger and nonmotileb. What are the tin y spheres inside the larger sphere of Volvox? daughter coloniesc. How do you suppose they get out? Parent colonies turn inside out or rupture.d. How do you think the number of cells in a young Volvox colony compares to the number in a mature colony? less than or equal to the number in the mature colony6.a. How is Fucus' structure different from green algae that you have examined earlier in this exercise? Fucus is larger, more parenchymatous, and flattened.b. Are all portions of the thallus photosynthetic?How can you tell? probably not, due to different concentrations of pigmentsc. Considering where Fucus lives, what do you think is the function of its gelatinous sheath? Since Fucus lives in intertidal regions, the gelatinous sheath probably prevents desiccation.d. Are the swollen structures solid masses or are they empty? essentially empty7.a. Are the gametes of Fucus isogamous or oogamous? oogamousb. How does the structure of tissue surrounding the reproductive structures compare w ith that of green algae? Tissues in brown algae are more complex.8. a. Can you see any pores in the walls of diatoms? robably yesb. Are any of the diatoms moving? possiblyc. If diatoms lack flagella, how do you explain their motility? gliding on a gelatinous secretiond. How would diatomaceous earth compare to sand as a swimming pool filter material? Which would be better and why? Diatomaceous earth would be better because it is finer. How do the shapes of dinoflagellates compare with other unicellular algae that you have observed in this exercise? bizarre and varied What is the function of the eyespot of Euglena? detect lightExercise 25 SURVEY OF KINGDOM PROTISTA: PROTOZOA AND SLIME MOLDS ANSWERS TO QUESTIONS1. a. Can you detect moving cytoplasm in the extending pseudopods of Amoeba? probably yesb. What do you suppose the Amoeba is moving toward or away from? light or foodc. How does the Amoeba respond to nutrient broth? usually increased movementd. Approximately how long would it t ake an Amoeba to move across the field of view on low power? a few minutese. Why is a contractile vacuole of a protozoan often more difficult to see than a food vacuole? A contractile vacuole contains clear excretory fluid, while food vacuoles contain particulate matter.f. Why would excess water tend to accumulate in Amoeba? ecause solute concentration in the amoeba is higher than the concentration in the environment and water diffuses into the cell2. How could fossilized forams in different geological layers of rock or sediment indicate the probability of finding oil? Different species are present in different environmental conditions; therefore, a list of species may include those associated with environmental conditions appropriate for oil formation.3. a. How large is a trypanosome relative to an Amoeba? much smallerb. What alga does a trypanosome superficially resemble? Euglena4. a. Are cilia visible on living or prepared Paramecium? probably notb.Does Paramecium rotate as it mo ves? yesc. How does movement of Paramecium compare with that of Amoeba? With a flagellated alga? Paramecium moves much faster than Amoeba, but not as fast as many flagellated species.5. a. Why is the division of Paramecium cells called â€Å"transverse† fission? The plane of separation is transverse to the longitudinal axis.b. Why is transverse fission not a sexual process? it doesn't recombine genesc. What are the advantages and disadvantages of conjugation in Paramecium? disadvantages: conjugation requires more than one organism and is energy consuming advantage: creation of new genetic combinations6. a. What is the value or function of the rapid contraction stalk of Vorticella? avoid predationb. What is the probable function of the moving cilia of Vorticella? create water currents bringing food particles to the organism7. a. Is cytoplasmic movement of Physarum apparent? probably yesb. Is the movement in a particular direction? probably not, but on moist medium they may app arently move toward foodc. What is a possible function of cytoplasmic movement in Physarum? avoid light, find foodExercise 26 SURVEY OF KINGDOM FUNGI: MOLDS, SAC FUNGI, MUSHROOMS, AND LICHENS ANSWERS TO QUESTIONS1. a. Are hyphae present? ob. Are the cells motile? yes2. a. How many species of mold are on the bread? 2, 3, or 4b. Do any of the molds on the bread have hyphae modified as sporangiophores and sporangia? probably yesc. Is pigment distributed uniformly in each mycelium? If not, where is the pigment concentrated in each mold? No, pigment is usually concentrated in sporangia.d. What is the adaptive significance of spores forming on ends of upright filaments rather than closer to the protective substrate? to enhance distribution of released spores3. a. In what structure is the dark pigment of Rhizopus concentrated? sporangiab.Is Rhizopus reproducing sexually as well as asexually in the same petri dish? How can you tell? Yes (if students are provided with cultures with two strai ns); because sporangia as well as zygospores are visible.4. What is the relative size of Penicillium hyphae compared to Rhizopus hyphae? Penicillium hyphae are much smaller than Rhizopus hyphae.5. a. Do you see chains of yeast cells produced by budding? probably yesb. How is the structure of yeast hyphae different from that of molds? Yeast hyphae are greatly reduced to a chain of one or two cells, while molds are very long and multicellular. 6. What is the difference between dikaryotic and diploid cells? Dikaryotic cells have two distinct nuclei, each with a haploid (single) set of chromosomes; a diploid cell has one nucleus with a diploid (double set of chromosomes).7. How many spores would you estimate are present on the gills of a single cap of Coprinus? Remember that a prepared slide shows only a cross section. many, many thousands8. a. What is the value of photosynthetic algae to the growth of a fungus in a lichen? Algae conduct photosynthesis and produce an energy rich carbon source, (i. e. , glucose).b. Would you expect lichens to grow best in rural or urban environments? Why? best in rural environments because they are so sensitive to air pollutionExercise 27 SURVEY OF THE PLANT KINGDOM: LIVERWORTS, MOSSES, AND HORNWORTS OF PHYLA HEPATICOPHYTA, BRYOPHYTA, AND ANTHOCEROPHYTA ANSWERS TO QUESTIONS1. What are the functions of rhizoids? anchorage, possibly absorption2. What is the function of these pores? gas exchange3. How do the positions of the archegonium and antheridium relate to their reproductive function? Antheridia need to disperse sperm and are upright with pores on top of the antheridial disk; archegonia are protected under the surface of the archegonial disk.4. a. What is the function of the foot? nutrient transport from gametophyte to sporophyteb. Are spores haploid or diploid? haploidc. What is the functional significance of the response of elaters to moisture? dispersal of spores5. a. How many cells thick is the leaflet? one or twob. Is there a midrib? Vein? yesc. Are stomata or pores visible on the leaf surface? probably notd. How does the symmetry of a moss gametophyte compare with that of a liverwort gametophyte? Mosses are radially symmetrical and liverworts are bilaterally symmetrical. 6. a. How many times its own weight did the moss absorb? or more timesb. How does this compare with the paper towel? much greaterc. Why is Sphagnum often used in shipping items that must be kept moist? because it holds water so well7. Where is the egg located in the archegonium? egg is enlarged cell at base of neck8. Are sperm haploid or diploid? haploid9. Is the sporophyte more prominent in mosses or liverworts? mosses10. What is the adaptive significance of the seta of the sporophyte growing well above the mat of the gametophyte? better dispersal11. a. What process produces spores? meiosisb. Is the capsule haploid or diploid? diploid12. Can you think of any evolutionary implications of the similarity between a moss protonema and a filamentous green alga? both have similar form and live in similar habitat, which suggests that selective pressures for developing these structures may have been similarExercise 28 SURVEY OF THE PLANT KINGDOM: SEEDLESS VASCULAR PLANTS OF PHYLA PTEROPHYTA, LYCOPHYTA, PSILOPHYTA, AND SPHENOPHYTA ANSWERS TO QUESTIONS1.a. Which parts of the life cycle are haploid? gametophyteb. Which are diploid? sporophyte2.a. How many veins are present in each frond? usually 1 large veinb. What tissues compose a vein? ylem and phloemc. What is the function of the stalk? The blade? The pinnae? stalk—support; blade and pinnae—photosynthesis3.a. What is the function of an annulus? breaking point for opening sporangiumb. Are any spores in the sporangium? probably so4.a. Did the application of acetone cause the spores of the fern to disperse? probably yesb. How is the mechanism for spore dispersal in ferns similar to that of bryophytes? Moisture causes changes in cell (elater or annulus) shap e to release spores.5.a. Is the prothallium haploid or diploid? haploidb. Is the prothallium sporophyte or gametophyte? gametophyte6. a.What is the adaptive significance of having these structures on the lower surface of the prothallium rather than on the upper surface? protection from desiccationb. What is the adaptive significance of having sperm and egg produced at different times? discourages inbreeding7. How do Salvinia and Azolla differ from other ferns you've examined earlier? smaller, aquatic8. a. What type of branching characterizes Psilotum? dichotomousb. Are any roots present? noc. Are any leaves present? nod. Where are the sporangia? at end of short lateral branchese. Where does photosynthesis occur in Psilotum? stem9.a. Where are the leaves? n rings along stemb. What part of the plant is photosynthetic? stemc. Which part of the life cycle of Equisetum is dominant, the sporophyte or gametophyte? sporophyte10.a. How does strobili formation in Equisetum compare with Lycopo dium and Selaginella? sporangia of Lycopodium and Selaginella are on microphyllsb. How do elaters aid in the dispersal of spores? moves spores11. a. How could a rhizome be involved in asexual reproduction? produces new growth, which could be separated from parent plant (e. g. , via fragmentation), thereby resulting in a new individualb. How is a rhizome different from a rhizoid? hizome is underground stem; rhizoid is small, rootlike structurec. Does the rhizome have leaves? depends on specimen, but probably yesd. What is the shape and size of the leaves? thin, elongate, arranged in spiralse. What is the significance of this form of the leaves? intercept light for photosynthesisf. Is a midvein visible? yes g. What does the word â€Å"evergreen† mean? does not lose all of its leaves seasonallyh. Is â€Å"evergreenness† a good characteristic for classifying plants? Why or why not? No, unrelated plants are evergreen.12. a. How many sporangia occur on each sporophyll of Lyc opodium? oneb.Can you see why spores of Lycopodium are sometimes called â€Å"vegetable sulfur†? yesc. Why are the spores a good, dry lubricant? powdery; certain species produce some oild. Which is the dominant part of the Lycopodium life cycle, the sporophyte or gametophyte? sporophyte13. a. Are spores of Selaginella similar in size? nob. What is this condition called? heterosporousc. What is the functional significance of the difference in the appearance of dehydrated and rehydrated Selaginella? water conservation and survival during droughtd. Can you see why these plants are sometimes referred to as â€Å"resurrection plants†? yes Mitosis Replication of Eukaryotic Cells Exercise 13MITOSIS: REPLICATION OF EUKARYOTIC CELLS  ANSWERS TO QUESTIONS1.a. Mitosis and cytokinesis are often referred to collectively as â€Å"cellular division. † Why are they more accurately called cellular replication? The result of mitosis is production of two cells (replicates) identical to the parent cell. The genetic material is replicated rather than divided.b. Does the cell cycle have a beginning and an end? The organization of our study of cellular events indicates a beginning and end, but the events continue to repeat.2.a. If a nucleus has eight chromosomes during interphase, how many chromosomes does it have during metaphase? During metaphase there are 8 double-stranded chromosomes.b. How many does it have after mitosis is complete? After mitosis (and separation of chromatids) there are 16 chromosomes distributed between two nuclei.3.a. Why would we choose an embryonic mass of cells for procedure 13. 3 in which to study the stages of mitosis? embryonic cells a re replicating and growing rapidlyb. Which stage of mitosis most often is associated with the beginning of cytokinesis? usually late anaphase4.a. What region of a root has the most mitotic activity? meristem, just above capb. Why is pinching of the cytoplasm inadequate for cytokinesis in plant cells? Pinching of cytoplasm is not appropriate for rigid cell walls.c. Locate a plant cell in late telophase. What is the volume of the new cells relative to a mature cell? Each cell is smaller (approximately 1/2) than the parent cell.5.a. Why are the combined data from all the class members more meaningful than your results alone? Combined results reduce random error.b. How accurate were your predictions for length of each stage of mitosis? probably not closec. What sources of error can you list for this technique to determine the time elapsed during each stage of mitosis? ariation in onion roots; student's ability to distinguish stages; microscopic cross sections of roots may or may not pas s through the nuclear materialExercise 14MEIOSIS: REDUCTION DIVISION AND GAMETOGENESIS ANSWERS TO QUESTIONS1. a. Why would shuffling genetic material and producing new combinations of characteristics be advantageous to a species? New genetic combinations allow adaptation to changing environments.b. When would it be deleterious? New combinations always produce a percentage of disadvantageous characteristics; the risk of bad gene combinations may be inappropriate in a stable, beneficial environment.2. a.Synapsis occurs after chromosomal DNA has replicated. How many chromatids are involved in crossing-over of a homologous pair of chromosomes?b. Suppose synapsis occurred between two homologous chromosomes, and one had alleles for blue eyes and brown hair and the other had alleles for green eyes and blonde hair. How many different combinations of these alleles would be possible? 4 Remember that crossing-over does not necessarily occur between the loci, so the original combinations may re main intact. 3. a. If a nucleus has eight chromosomes when it begins meiosis, how many chromosomes does it have after telophase I? Telophase II? with two chromatids each; 4 with one chromatid eachb. What are the major differences between the events of meiosis and mitosis? (see table 14. 2)c. What are some minor differences, and why do you consider them minor? minor differences would be those unrelated to chromosomal events4. a. During gametogenesis a sperm cell undergoes considerable structural change. What are the basics of sperm structure and how does it relate to function? The basics include small head with nucleus; an acrosomal process with enzymes to digest the outer membranes of the egg; a head and neck rich in mitochondria; and tail used to propel the cell to the egg.b. What is the advantage of producing sperm in a system of tubes rather than in solid tissue? because sperm cells can be expelled easily from the body through a system of tubesc. What is each strand of a double-s tranded chromosome called? chromatid5. How would retaining extra cytoplasm enhance survival of a developing oocyte? More cytoplasm contains more nutrients to ensure survival.6. a. What are the relative sizes of oocytes in a dormant follicle, a growing follicle, and a Graafian follicle? dormant oocyte 1X, growing oocyte 5X, mature oocyte 25Xb. Are polar bodies visible in your prepared slide of a cat ovary? Why or why not? probably not, because cells in the ovary usually have not finished meiosis I, and polar bodies disintegrate rapidly DNA FINGERPRINTING LABRestriction Digestion of DNA Samples Observation QuestionsDescribe the samples of DNA (physical properties). The DNA samples are clear, colorless liquid samples.Is there any observable difference between the samples of DNA? No. All samples appear similar.Describe the appearance of the restriction endonuclease mix. The restriction enzymes appear to be clear, colorless liquids.Restriction Digestion of DNA Samples Review QuestionsBef ore you incubated your samples, describe any visible signs of change in the contents of the tubes containing the DNA combined with the restriction enzymes. DNA + EcoRI/PstI enzyme mix: No visible change apparent in the tubes.Can you see any evidence to indicate that your samples of DNA were fragmented or altered in any way by the addition of EcoRI/PstI? Explain. No. No visible change apparent in the tubes.In the absence of visible evidence of change, is it still possible that the DNA samples were fragmented? Explain your reasoning. Yes. They may be chemically changed but the changes may not be visible. Enzymes may have cut the DNA.After a 24 hour incubation period, are there any visible clues that the restriction enzymes may have in some way changed the DNA in any of the tubes? Explain your reasoning. No. No visible change apparent in the tubes but the enzymes may have cut the DNA. The reactions are at the molecular level and too small to be seen.Electrophoresis of Your DNA Samples Review QuestionsThe electrophoresis apparatus creates an electrical field [positive and negative ends of the gel]. DNA molecules are negatively charged. To which pole of the electrophoresis field would you expect DNA to migrate (+ or -)? Explain. Positive.What color represents the negative pole? Black.After DNA samples are loaded in wells, they are â€Å"forced† to move through the gel matrix. Which size fragment (large vs small) would you expect to move toward the opposite end of the gel most quickly? Explain. Smaller. There is less resistance to their movement through the gel matrix.Which fragments are expected to travel the shortest distance [remain closest to the well]? Explain. Larger. There is more resistance to their movement through the gel matrix.Lesson 4 Thought QuestionsWhat can you assume is contained within each band? DNA fragments.If this were a fingerprinting gel, then how many kinds (samples) of DNA can you assume were placed in each separate well? One.What wo uld be a logical explanation as to why there is more than one band of DNA for each of the samples? The DNA must have been cut into fragments by restriction enzymes.What probably caused the DNA to become fragmented? The chemical action of the restriction enzymes cutting at specific base sequences.Which of the DNA samples have the same number of restriction sites for the restriction endonuclease used? Write the lane numbers.Lanes 2, 3, and 4 (CS, S1, and S2).Which sample has the smallest DNA fragment? The sample in lane 5 (S3).How many restriction sites were there in lane three? Two sites that cut the sample into two fragments.Which DNA samples appear to have been â€Å"cut† into the same number and size of fragments? Lanes 2 and 4 (CS and S2).Based on your analysis of the photograph, what is your conclusion about the DNA samples in the photograph? Do any of the samples seem to be from the same source. If so which ones? Describe the evidence that supports your conclusion.The DN A samples in lanes 2 and 4 (CS and S2) are from the same individual because they have identical restrictions sites that yield identical fragments.DNA Fingerprinting: OverviewWhat are we trying to determine? Restate the central question. We are trying to determine if samples of DNA that we were provided with are from the same individual or from different individuals.Which of your DNA samples were fragmented? What would your gel look like if the DNA were not fragmented? The number of fragmented samples will vary. They will have one band on the gel if the DNA was not cut.What caused the DNA to become fragmented? The addition of restriction enzymes.What determines where a restriction endonuclease will â€Å"cut† a DNA molecule? A special sequence of bases on the DNA called restriction sites.A restriction endonuclease â€Å"cuts† two DNA molecules at the same location. What can you assume is identical about the molecules at that location? The restriction sites are identical .Do any of your suspect samples appear to have EcoRI or PstI recognition sites at the same location as the DNA from the crime scene? The samples in lanes 2 and 5 match (CS and S3).Based on the above analysis, do any of the suspect samples of DNA seem to be from the same individual as the DNA from the crime scene? Describe the scientific evidence that supports your conclusion. The CS and S3 samples appear to be identical. They both produce similar banding patterns on the gel.Exercise 23 SURVEY OF BACTERIA: KINGDOMS ARCHAEBACTERIA AND BACTERIA ANSWERS TO QUESTIONS1. a. Why is it important that bacteria release nutrients? Nutrients must be released and recycled for use by other living organisms. If nutrients were not released by decomposers, the nutrients would all eventually be locked up in dead, non-decaying tissue.b. What term would best describe heterotrophic bacteria that feed on living tissue? parasitism2. What is the shape and size of each bacterial colony? E. coli—medium rod; B. megaterium—large rod; R. rubrum—small and thin spirillum 3. a. Which type of bacteria is most prevalent in the sample from your teeth? How do you know? probably gram positive because most of the cells are purpleb. Is Bacillus megaterium gram positive or gram negative? gram positive4. a. Where are the bacteria? Are they between cells or inside cells? inside the cellsb. Why is this relationship between a plant and bacterium called mutualism? Both partners benefit from the association.c. How does Rhizobium benefit from this association? nutrients from the hostd. How does the host plant benefit from the association? nitrogen supply from the bacterium5. Based on their appearance, which drugs or chemicals retard the growth of bacteria? depends on the drugs that you use6. Do all cells of a trichome of Oscillatoria appear similar? yes7. a. Do adjacent cells share a common sheath? usuallyb. What do you suppose is the function of the sheath? protectionc. Do clusters of Gloeocapsa represent multicellular organisms? Why or why not?No, the cells are not obligate dependents—they can operate independently; the protoplasms of the cells are not connected; the aggregation of cells can be broken into individual cells without killing the cells.8. a. How is the shape of Merismopedia different from other cyanobacteria you studied in this exercise? Merismopedia is a flat, square colony one cell thick.b. How would a colony attain this shape? equal divisions in two planesExercise 24Survey of Kingdom Protista: The Algae ANSWERS TO QUESTIONS1. a. Is the movement of Chlamydomonas smooth or does it appear jerky? relatively smoothb. Can you see both flagella? You may need to reduce the light intensity to see flagella. probably not, depends on student's microscopec. How does methylcellulose affect movement of Chlamydomonas? slows it downd. How does the stigma help Chlamydomonas survive? detects light2. a. Under what environmental conditions would a zygote not u ndergo meiosis immediately? unfavorable conditionsb. Are spores of Chlamydomonas haploid or diploid? haploidc. Which portions of the life cycle of Chlamydomonas are haploid? all portions from meiosis to syngamyd. Which are diploid? all portions from syngamy to meiosis3. a. Are the filaments of Spirogyra branched? nob. What is the shape of the chloroplasts of Spirogyra? spiralc. Can you see any conjugation tubes? If you can't, examine the prepared slides that demonstrate these structures. probably not seen on live material, but easily seen on prepared materiald. How do you think that Spirogyra reproduces asexually? mitosis and fragmentation4. a. How is Cladophora morphologically similar to Spirogyra. How is it different? They are both filaments, but Cladophora is branched and Spirogyra is unbranched.b. What is the shape of its chloroplasts? broad and dispersed5. a. What is oogamy? ne gamete (sperm) is small and motile, the other gamete (egg) is larger and nonmotileb. What are the tin y spheres inside the larger sphere of Volvox? daughter coloniesc. How do you suppose they get out? Parent colonies turn inside out or rupture.d. How do you think the number of cells in a young Volvox colony compares to the number in a mature colony? less than or equal to the number in the mature colony6.a. How is Fucus' structure different from green algae that you have examined earlier in this exercise? Fucus is larger, more parenchymatous, and flattened.b. Are all portions of the thallus photosynthetic?How can you tell? probably not, due to different concentrations of pigmentsc. Considering where Fucus lives, what do you think is the function of its gelatinous sheath? Since Fucus lives in intertidal regions, the gelatinous sheath probably prevents desiccation.d. Are the swollen structures solid masses or are they empty? essentially empty7.a. Are the gametes of Fucus isogamous or oogamous? oogamousb. How does the structure of tissue surrounding the reproductive structures compare w ith that of green algae? Tissues in brown algae are more complex.8. a. Can you see any pores in the walls of diatoms? robably yesb. Are any of the diatoms moving? possiblyc. If diatoms lack flagella, how do you explain their motility? gliding on a gelatinous secretiond. How would diatomaceous earth compare to sand as a swimming pool filter material? Which would be better and why? Diatomaceous earth would be better because it is finer. How do the shapes of dinoflagellates compare with other unicellular algae that you have observed in this exercise? bizarre and varied What is the function of the eyespot of Euglena? detect lightExercise 25 SURVEY OF KINGDOM PROTISTA: PROTOZOA AND SLIME MOLDS ANSWERS TO QUESTIONS1. a. Can you detect moving cytoplasm in the extending pseudopods of Amoeba? probably yesb. What do you suppose the Amoeba is moving toward or away from? light or foodc. How does the Amoeba respond to nutrient broth? usually increased movementd. Approximately how long would it t ake an Amoeba to move across the field of view on low power? a few minutese. Why is a contractile vacuole of a protozoan often more difficult to see than a food vacuole? A contractile vacuole contains clear excretory fluid, while food vacuoles contain particulate matter.f. Why would excess water tend to accumulate in Amoeba? ecause solute concentration in the amoeba is higher than the concentration in the environment and water diffuses into the cell2. How could fossilized forams in different geological layers of rock or sediment indicate the probability of finding oil? Different species are present in different environmental conditions; therefore, a list of species may include those associated with environmental conditions appropriate for oil formation.3. a. How large is a trypanosome relative to an Amoeba? much smallerb. What alga does a trypanosome superficially resemble? Euglena4. a. Are cilia visible on living or prepared Paramecium? probably notb.Does Paramecium rotate as it mo ves? yesc. How does movement of Paramecium compare with that of Amoeba? With a flagellated alga? Paramecium moves much faster than Amoeba, but not as fast as many flagellated species.5. a. Why is the division of Paramecium cells called â€Å"transverse† fission? The plane of separation is transverse to the longitudinal axis.b. Why is transverse fission not a sexual process? it doesn't recombine genesc. What are the advantages and disadvantages of conjugation in Paramecium? disadvantages: conjugation requires more than one organism and is energy consuming advantage: creation of new genetic combinations6. a. What is the value or function of the rapid contraction stalk of Vorticella? avoid predationb. What is the probable function of the moving cilia of Vorticella? create water currents bringing food particles to the organism7. a. Is cytoplasmic movement of Physarum apparent? probably yesb. Is the movement in a particular direction? probably not, but on moist medium they may app arently move toward foodc. What is a possible function of cytoplasmic movement in Physarum? avoid light, find foodExercise 26 SURVEY OF KINGDOM FUNGI: MOLDS, SAC FUNGI, MUSHROOMS, AND LICHENS ANSWERS TO QUESTIONS1. a. Are hyphae present? ob. Are the cells motile? yes2. a. How many species of mold are on the bread? 2, 3, or 4b. Do any of the molds on the bread have hyphae modified as sporangiophores and sporangia? probably yesc. Is pigment distributed uniformly in each mycelium? If not, where is the pigment concentrated in each mold? No, pigment is usually concentrated in sporangia.d. What is the adaptive significance of spores forming on ends of upright filaments rather than closer to the protective substrate? to enhance distribution of released spores3. a. In what structure is the dark pigment of Rhizopus concentrated? sporangiab.Is Rhizopus reproducing sexually as well as asexually in the same petri dish? How can you tell? Yes (if students are provided with cultures with two strai ns); because sporangia as well as zygospores are visible.4. What is the relative size of Penicillium hyphae compared to Rhizopus hyphae? Penicillium hyphae are much smaller than Rhizopus hyphae.5. a. Do you see chains of yeast cells produced by budding? probably yesb. How is the structure of yeast hyphae different from that of molds? Yeast hyphae are greatly reduced to a chain of one or two cells, while molds are very long and multicellular. 6. What is the difference between dikaryotic and diploid cells? Dikaryotic cells have two distinct nuclei, each with a haploid (single) set of chromosomes; a diploid cell has one nucleus with a diploid (double set of chromosomes).7. How many spores would you estimate are present on the gills of a single cap of Coprinus? Remember that a prepared slide shows only a cross section. many, many thousands8. a. What is the value of photosynthetic algae to the growth of a fungus in a lichen? Algae conduct photosynthesis and produce an energy rich carbon source, (i. e. , glucose).b. Would you expect lichens to grow best in rural or urban environments? Why? best in rural environments because they are so sensitive to air pollutionExercise 27 SURVEY OF THE PLANT KINGDOM: LIVERWORTS, MOSSES, AND HORNWORTS OF PHYLA HEPATICOPHYTA, BRYOPHYTA, AND ANTHOCEROPHYTA ANSWERS TO QUESTIONS1. What are the functions of rhizoids? anchorage, possibly absorption2. What is the function of these pores? gas exchange3. How do the positions of the archegonium and antheridium relate to their reproductive function? Antheridia need to disperse sperm and are upright with pores on top of the antheridial disk; archegonia are protected under the surface of the archegonial disk.4. a. What is the function of the foot? nutrient transport from gametophyte to sporophyteb. Are spores haploid or diploid? haploidc. What is the functional significance of the response of elaters to moisture? dispersal of spores5. a. How many cells thick is the leaflet? one or twob. Is there a midrib? Vein? yesc. Are stomata or pores visible on the leaf surface? probably notd. How does the symmetry of a moss gametophyte compare with that of a liverwort gametophyte? Mosses are radially symmetrical and liverworts are bilaterally symmetrical. 6. a. How many times its own weight did the moss absorb? or more timesb. How does this compare with the paper towel? much greaterc. Why is Sphagnum often used in shipping items that must be kept moist? because it holds water so well7. Where is the egg located in the archegonium? egg is enlarged cell at base of neck8. Are sperm haploid or diploid? haploid9. Is the sporophyte more prominent in mosses or liverworts? mosses10. What is the adaptive significance of the seta of the sporophyte growing well above the mat of the gametophyte? better dispersal11. a. What process produces spores? meiosisb. Is the capsule haploid or diploid? diploid12. Can you think of any evolutionary implications of the similarity between a moss protonema and a filamentous green alga? both have similar form and live in similar habitat, which suggests that selective pressures for developing these structures may have been similarExercise 28 SURVEY OF THE PLANT KINGDOM: SEEDLESS VASCULAR PLANTS OF PHYLA PTEROPHYTA, LYCOPHYTA, PSILOPHYTA, AND SPHENOPHYTA ANSWERS TO QUESTIONS1.a. Which parts of the life cycle are haploid? gametophyteb. Which are diploid? sporophyte2.a. How many veins are present in each frond? usually 1 large veinb. What tissues compose a vein? ylem and phloemc. What is the function of the stalk? The blade? The pinnae? stalk—support; blade and pinnae—photosynthesis3.a. What is the function of an annulus? breaking point for opening sporangiumb. Are any spores in the sporangium? probably so4.a. Did the application of acetone cause the spores of the fern to disperse? probably yesb. How is the mechanism for spore dispersal in ferns similar to that of bryophytes? Moisture causes changes in cell (elater or annulus) shap e to release spores.5.a. Is the prothallium haploid or diploid? haploidb. Is the prothallium sporophyte or gametophyte? gametophyte6. a.What is the adaptive significance of having these structures on the lower surface of the prothallium rather than on the upper surface? protection from desiccationb. What is the adaptive significance of having sperm and egg produced at different times? discourages inbreeding7. How do Salvinia and Azolla differ from other ferns you've examined earlier? smaller, aquatic8. a. What type of branching characterizes Psilotum? dichotomousb. Are any roots present? noc. Are any leaves present? nod. Where are the sporangia? at end of short lateral branchese. Where does photosynthesis occur in Psilotum? stem9.a. Where are the leaves? n rings along stemb. What part of the plant is photosynthetic? stemc. Which part of the life cycle of Equisetum is dominant, the sporophyte or gametophyte? sporophyte10.a. How does strobili formation in Equisetum compare with Lycopo dium and Selaginella? sporangia of Lycopodium and Selaginella are on microphyllsb. How do elaters aid in the dispersal of spores? moves spores11. a. How could a rhizome be involved in asexual reproduction? produces new growth, which could be separated from parent plant (e. g. , via fragmentation), thereby resulting in a new individualb. How is a rhizome different from a rhizoid? hizome is underground stem; rhizoid is small, rootlike structurec. Does the rhizome have leaves? depends on specimen, but probably yesd. What is the shape and size of the leaves? thin, elongate, arranged in spiralse. What is the significance of this form of the leaves? intercept light for photosynthesisf. Is a midvein visible? yes g. What does the word â€Å"evergreen† mean? does not lose all of its leaves seasonallyh. Is â€Å"evergreenness† a good characteristic for classifying plants? Why or why not? No, unrelated plants are evergreen.12. a. How many sporangia occur on each sporophyll of Lyc opodium? oneb.Can you see why spores of Lycopodium are sometimes called â€Å"vegetable sulfur†? yesc. Why are the spores a good, dry lubricant? powdery; certain species produce some oild. Which is the dominant part of the Lycopodium life cycle, the sporophyte or gametophyte? sporophyte13. a. Are spores of Selaginella similar in size? nob. What is this condition called? heterosporousc. What is the functional significance of the difference in the appearance of dehydrated and rehydrated Selaginella? water conservation and survival during droughtd. Can you see why these plants are sometimes referred to as â€Å"resurrection plants†? yes